ArticleFrontiers in bioengineering and biotechnology2023
Simple method for quantification of anionic biosurfactants in aqueous solutions.
Article in Frontiers in bioengineering and biotechnology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 6 citations in OpenAlex.
- Unlocking the Synergistic Potential and Efficacy of Biosurfactant-Silver Nanoparticle for Enhanced Antimicrobial Activities.Molecular biotechnology · 2026Article
- Assessing hydrocarbon degradation capacity ofFrontiers in microbiology · 2025Article
- Rhamnolipids bio-production and miscellaneous applications towards green technologies: a literature review.PeerJ · 2025Review
- Anti-Fungal (Pathogens (Basel, Switzerland) · 2024Article
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Authors and funding
4 authors at 3 institutions in 2 countries.
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No grant is acknowledged in the PubMed record.
Abstract
Biosurfactants are microbial products that have applications as cleaning agents, emulsifiers, and dispersants. Detection and quantification of biosurfactants can be done by various methods, including colorimetric tests, high performance liquid chromatography (HPLC) coupled to several types of detectors, and tests that take advantage of biosurfactants reducing surface tension of aqueous liquids, allowing for spreading and droplet formation of oils. We present a new and simple method for quantifying biosurfactants by their ability, on paper, to reduce surface tension of aqueous solutions, causing droplet dispersion on an oiled surface in correlation with biosurfactant content. We validated this method with rhamnolipids, surfactin, sophorolipids, and ananatoside B; all are anionic microbial surfactants. Linear ranges for quantification in aqueous solutions for all tested biosurfactants were between 10 and 500 µM. Our method showed time-dependent biosurfactant accumulation in cultures of
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