ReviewFrontiers in bioengineering and biotechnology2023
Advancements in the synergy of isothermal amplification and CRISPR-cas technologies for pathogen detection.
Review in Frontiers in bioengineering and biotechnology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed, 45 citations in OpenAlex.
- One-Pot RPA-CRISPR/Cas12a Assay With Visual Readout for the Ultra-Specific Detection of Monkeypox Virus Clade I.Microbial biotechnology · 2026Article
- Advancements in CRISPR/Cas Technologies for Sensitive Cancer Detection: Mechanisms, Platforms, and Clinical Translation Roadmap.Diagnostics (Basel, Switzerland) · 2026Review
- A Rapid, Field-Deployable Diagnostic Platform for Getah Virus Based on RT-RAA and CRISPR EsCas13d.Microbial biotechnology · 2026Article
- A one-pot biplex RPA-Cas assay for sensitive detection of Mycobacterium tuberculosis from tongue swabs.Scientific reports · 2026Article
- Programmable AND-gate strategy to reduce false positives in rolling circle amplification.Biosensors & bioelectronics · 2026Article
- Development of a Single-Tube Asymmetric ERA-CRISPR/Cas12a Assay for Rapid Visual Detection ofMicroorganisms · 2026Article
- A temporally controlled isothermal amplification-CRISPR/Cas12a platform for the rapid detection of monkeypox virus.Virology journal · 2026Article
- Rapid Detection of Chicken Infectious Anemia Virus Using a One-Tube RPA-CRISPR/Cas12a System.Veterinary sciences · 2026Article
- The evolution of next-generation lateral flow assays for bacterial and fungal diagnostics.Mikrochimica acta · 2026Review
- Rapid and Simple Detection of Enterocytozoon Bieneusi Using Lateral Flow Assay Based on Recombinase Polymerase Amplification or Nested PCR Combined with CRISPR-Cas12a.Acta parasitologica · 2026Article
- Establishment of a One-Step Rapid Visual Detection Method for Pigeon Circovirus Based on the RAA-CRISPR/Cas12a Assay.Veterinary sciences · 2026Article
- Single Isothermal Assay for Multi-Site Mutation Detection of Rifampicin Resistance inPathogens (Basel, Switzerland) · 2026Article
- Construction of an RPA-CRISPR visualization system for rapid detection of Burkholderia mallei.BMC microbiology · 2026Article
- An overview of CRISPR-artificial intelligence theranostics: Current and emerging applications.Biomaterials translational · 2026Review
- Development and evaluation of a recombinase-aided amplification-lateral flow dipstick assay for rapid and sensitive detection ofFrontiers in cellular and infection microbiology · 2026Article
- Future Research Directions on Mycobacterium tuberculosis Proteins.Advances in experimental medicine and biology · 2026Review
- Integrated Colorimetric CRISPR/Cas12a Detection of Double-Stranded DNA on Microfluidic Paper-Based Analytical Devices.Biosensors · 2026Article
- Rapid and Simple Detection ofPathogens (Basel, Switzerland) · 2025Article
- Article
- Molecular diagnosis of Trichinella spp.: current status and future prospects.Parasitology research · 2025Review
Corrections and comments
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Authors and funding
9 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the realm of pathogen detection, isothermal amplification technology has emerged as a swift, precise, and sensitive alternative to conventional PCR. This paper explores the fundamental principles of recombinase polymerase amplification (RPA) and recombinase-aid amplification (RAA) and reviews the current status of integrating the CRISPR-Cas system with RPA/RAA techniques. Furthermore, this paper explores the confluence of isothermal amplification and CRISPR-Cas technology, providing a comprehensive review and enhancements of existing combined methodologies such as SHERLOCK and DETECTR. We investigate the practical applications of RPA/RAA in conjunction with CRISPR-Cas for pathogen detection, highlighting how this integrated approach significantly advances both research and clinical implementation in the field. This paper aims to provide readers with a concise understanding of the fusion of RPA/RAA and CRISPR-Cas technology, offering insights into their clinical utility, ongoing enhancements, and the promising prospects of this integrated approach in pathogen detection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.