ArticleNature methods2023
Pulsed stimulated Brillouin microscopy enables high-sensitivity mechanical imaging of live and fragile biological specimens.
Article in Nature methods, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Nuclear mechanotransduction: tools for mechanical perturbation and chromatin characterization.Nucleus (Austin, Tex.) · 2026Review
- Multiscale Architecture and Mechanics of the Cell Nucleus: Implications for Disease, Bioengineering and Nanomedicine.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Brillouin microscopy for contact- and label-free quantification of tissue stiffness in lung fibrosis.American journal of respiratory cell and molecular biology · 2026Article
- Wavelet time-frequency analysis enhances noise-resilient quantification of corneal wave velocity and natural frequency in optical coherence elastography.Biomedical optics express · 2026Article
- From biomolecular condensates to functional nanomaterials: LLPS-inspired frameworks for nanoscale hydrogels and adaptive materials.Journal of nanobiotechnology · 2026Review
- Macrophages Mediate Mesoscale Brain Mechanical Homeostasis.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Review
- Cellular mechanical properties in response to environmental viscosity imaged by Brillouin Microscopy.Communications biology · 2025Article
- Mapping single-cell rheology of ascidian embryos in the cleavage stages using AFM.Biophysical journal · 2025Article
- Laser-emission vibrational microscopy of microdroplet arrays for high-throughput screening of hyperlipidemia.Light, science & applications · 2025Article
- NaBC1 Boron Transporter Enables Myoblast Response to Substrate Rigidity via Fibronectin-Binding Integrins.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Measuring and manipulating mechanical forces during development.Nature cell biology · 2025Review
- Improvement of gain and spatial resolution for impulsive stimulated Brillouin scattering microscopy.Photoacoustics · 2025Article
- How to Study the Mechanobiology of Intestinal Epithelial Organoids? A Review of Culture Supports, Imaging Techniques, and Analysis Methods.Biology of the cell · 2025Review
- The mechanobiology of biomolecular condensates.Biophysics reviews · 2025Review
- Remodeling of extracellular matrix collagen IV by MIG-6/papilin regulates neuronal architecture.Research square · 2025Article
- Biomechanics in the tumor microenvironment: from biological functions to potential clinical applications.Experimental hematology & oncology · 2025Review
- Full-field Brillouin microscopy based on an imaging Fourier-transform spectrometer.Nature photonics · 2025Article
- Harnessing quantum light for microscopic biomechanical imaging of cells and tissues.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Impact of polarization pulling on optimal spectrometer design for stimulated Brillouin scattering microscopy.APL photonics · 2024Article
Corrections and comments
- Erratum issued
Authors and funding
14 authors.
Funding
Abstract
Brillouin microscopy is an emerging optical elastography technique capable of assessing mechanical properties of biological samples in a three-dimensional, all-optical and noncontact fashion. The typically weak Brillouin scattering signal can be substantially enhanced via a stimulated Brillouin scattering (SBS) process; however, current implementations require high pump powers, which prohibit applications to photosensitive or live imaging of biological samples. Here we present a pulsed SBS scheme that takes advantage of the nonlinearity of the pump-probe interaction. In particular, we show that the required pump laser power can be decreased ~20-fold without affecting the signal levels or spectral precision. We demonstrate the low phototoxicity and high specificity of our pulsed SBS approach by imaging, with subcellular detail, sensitive single cells, zebrafish larvae, mouse embryos and adult Caenorhabditis elegans. Furthermore, our method permits observing the mechanics of organoids and C. elegans embryos over time, opening up further possibilities for the field of mechanobiology.
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