ArticleScientific reports2023
Abnormal centriolar biomarker ratios correlate with unexplained bull artificial insemination subfertility: a pilot study.
Article in Scientific reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Bovine In Vitro Fertilization Embryo Development Predicted by Capacitation-Induced Zinc Ion Flux and Acrosome Remodeling.International journal of molecular sciences · 2026Article
- The spermatozoon neck role in infertility and intracytoplasmic sperm injection outcomes.Journal of assisted reproduction and genetics · 2026Review
- POC1A colocalizes with POC1B in human and bovine spermatozoa centrioles.microPublication biology · 2026Article
- Spermatozoa centriole quality determined by FRAC may correlate with zygote nucleoli polarization-a pilot study.Journal of assisted reproduction and genetics · 2025Article
- CEP44 and CCDC15 label the spermatozoa proximal and atypical distal centrioles.microPublication biology · 2025Article
- Selecting sires to improve reproductive success: key traits for enhanced fertility and embryo development.Animal reproduction · 2025Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mechanisms underlying male infertility are poorly understood. Most mammalian spermatozoa have two centrioles: the typical barrel-shaped proximal centriole (PC) and the atypical fan-like distal centriole (DC) connected to the axoneme (Ax). These structures are essential for fertility. However, the relationship between centriole quality and subfertility (reduced fertility) is not well established. Here, we tested the hypothesis that assessing sperm centriole quality can identify cattle subfertility. By comparing sperm from 25 fertile and 6 subfertile bulls, all with normal semen analyses, we found that unexplained subfertility and lower sire conception rates (pregnancy rate from artificial insemination in cattle) correlate with abnormal centriolar biomarker distribution. Fluorescence-based Ratiometric Analysis of Sperm Centrioles (FRAC) found only four fertile bulls (4/25, 16%) had positive FRAC tests (having one or more mean FRAC ratios outside of the distribution range in a group's high-quality sperm population), whereas all of the subfertile bulls (6/6, 100%) had positive FRAC tests (P = 0.00008). The most sensitive biomarker was acetylated tubulin, which had a novel labeling pattern between the DC and Ax. These data suggest that FRAC and acetylated tubulin labeling can identify bull subfertility that remains undetected by current methods and may provide insight into a novel mechanism of subfertility.
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