Evidence map›Paper›PMID 37882869›Full record

ArticleArchivum immunologiae et therapiae experimentalis2023

Expression of Angiopoietin-2 in Lung Tissue of Juvenile SD Rats with Lipopolysaccharide-Induced Acute Lung Injury and the Role of Ulinastatin.

Junying Qiao, Shanshan Guo, Xianjie Huang, Luodan Zhang, Fan Li, Yazhen Fan

Open access · bronzeAbstract read
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In one paragraph

Article in Archivum immunologiae et therapiae experimentalis, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.6field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 4 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Junying QiaoDepartment of Pediatric Critical Care Medicine, The Third Affiliated Hospital of Zhengzhou University, No. 7 Kangfuqian Street, Zhengzhou, 450052, Henan, China. yingyingjun18@yeah.net.
Shanshan GuoDepartment of Pediatrics, The Third Clinical College of Academy of Medical Sciences, Zhengzhou University, Zhengzhou, 450052, China.
Xianjie HuangDepartment of Pediatric Critical Care Medicine, The Third Affiliated Hospital of Zhengzhou University, No. 7 Kangfuqian Street, Zhengzhou, 450052, Henan, China.
Luodan ZhangDepartment of Pediatric Critical Care Medicine, The Third Affiliated Hospital of Zhengzhou University, No. 7 Kangfuqian Street, Zhengzhou, 450052, Henan, China.
Fan LiDepartment of Pediatric Critical Care Medicine, The Third Affiliated Hospital of Zhengzhou University, No. 7 Kangfuqian Street, Zhengzhou, 450052, Henan, China.
Yazhen FanDepartment of Pediatric Critical Care Medicine, The Third Affiliated Hospital of Zhengzhou University, No. 7 Kangfuqian Street, Zhengzhou, 450052, Henan, China.
Third Affiliated Hospital of Zhengzhou University · CN

Funding

Henan Province Science and Technology Research Project 222102310191
6 · The paper itself

Abstract

This study aimed to observe the expression of angiopoietin-2 (Ang-2) in the lung tissue of juvenile SD rats with lipopolysaccharide (LPS)-induced acute lung injury (ALI) and to clarify the role of ulinastatin (UTI). Ninety 18-21-day-old juvenile SD male rats were randomly divided into five groups (n = 18). ALI rat model was established by intraperitoneal injection of LPS (LPS 10 mg/kg), while the control group was given the same dose of normal saline. The UTI intervention group was given the injection of UTI (5000 U/mL) immediately after the injection of LPS, which was divided into UTI low-dose group (LPS + 5 ml/kg UTI), UTI medium-dose group (LPS + 10 ml/kg UTI), and UTI high-dose group (LPS + 20 ml/kg UTI).The respiratory status of each group of rats was observed, and six rats were randomly selected to be killed in each group at 6, 12, and 24 h, and the lung tissues were dissected and retained. The pathological changes of the lung tissues were observed by hematoxylin-eosin (HE) staining, the expression levels and locations of Ang-2 and vascular endothelial growth factor (VEGF) in lung tissue were observed by immunohistochemical staining, and the expressions of genes and proteins of Ang-2 and VEGF were detected by quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blot analysis. Three hours after intraperitoneal injection, rats in the model group developed shortness of breath and the developed respiratory distress progressed over time. The lung pathological changes in the model group were obvious compared with those in the control group, and gradually worsened with time, and the pathological changes of lung in the rats in the UTI intervention group were reduced compared with those in the model group. At different time points, the expressions of Ang-2 and VEGF in the lung tissue of rats in the model group were higher than those in the control group, and were lower in the UTI intervention group than those in the model group. The expressions of Ang-2 and VEGF protein were lower in the low-dose group of UTI group than those in the high-dose group of UTI group at different time points (P < 0.05), and the expressions of Ang-2 and VEGF protein in the low-dose group of UTI were significantly lower than those in the medium-dose group at 12 h and 24 h (P < 0.05). The expression of Ang-2 was increased in the lung tissue of juvenile SD rats with LPS-induced ALI, and was associated with the degree of lung injury. UTI might attenuate LPS-induced ALI by inhibiting the expression of Ang-2 in lung tissue, and the low dose was more obvious than the medium and high dose.

Indexed as

Acute Lung InjuryAngiopoietin-2AnimalsGlycoproteinsLipopolysaccharidesLungMaleRatsRats, Sprague-DawleyVascular Endothelial Growth Factor AAngiopoietin-2GlycoproteinsLipopolysaccharidesurinastatinVascular Endothelial Growth Factor AAcute lung injuryAngiopoietin-2LipopolysaccharideRatsUlinastatinVEGF

Identifiers

PMID37882869
OpenAlexW4387950509

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.