Evidence map›Paper›PMID 37882455›Full record

ArticleBioconjugate chemistry2023

A Simple and Versatile Strategy for Oriented Immobilization of His-Tagged Proteins on Magnetic Nanoparticles.

Christian Castro-Hinojosa, Susel Del Sol-Fernández, Eduardo Moreno-Antolín, Beatriz Martín-Gracia, Jesús G Ovejero, Jesús Martínez de la Fuente, Valeria Grazú, Raluca M Fratila, María Moros

Open access · hybridAbstract read
In one paragraph

Article in Bioconjugate chemistry, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
2.0field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 20 citations in OpenAlex.

  1. Article
  2. Magnetically Recoverable ICT-Functionalized FeMolecules (Basel, Switzerland) · 2026
    Article
  3. Article
  4. Review
  5. Magnetic Cell Targeting for Cardiovascular Tissue Engineering.Tissue engineering. Part B, Reviews · 2025
    Review
  6. Review
  7. Antibody-Nanoparticle Conjugates in Therapy: Combining the Best of Two Worlds.Small (Weinheim an der Bergstrasse, Germany) · 2025
    Review
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Christian Castro-HinojosaInstituto de Nanociencia y Materiales de Aragón, INMA (CSIC-Universidad de Zaragoza), C/Pedro Cerbuna 12, Zaragoza 50009, Spain.
Susel Del Sol-FernándezInstituto de Nanociencia y Materiales de Aragón, INMA (CSIC-Universidad de Zaragoza), C/Pedro Cerbuna 12, Zaragoza 50009, Spain.
Eduardo Moreno-AntolínInstituto de Nanociencia y Materiales de Aragón, INMA (CSIC-Universidad de Zaragoza), C/Pedro Cerbuna 12, Zaragoza 50009, Spain.
Beatriz Martín-GraciaInstituto de Nanociencia y Materiales de Aragón, INMA (CSIC-Universidad de Zaragoza), C/Pedro Cerbuna 12, Zaragoza 50009, Spain.
Jesús G OvejeroInstituto de Ciencia de Materiales de Madrid (ICMM/CSIC), Sor Juana Inés de la Cruz 3, Madrid 28049, Spain.
Jesús Martínez de la FuenteInstituto de Nanociencia y Materiales de Aragón, INMA (CSIC-Universidad de Zaragoza), C/Pedro Cerbuna 12, Zaragoza 50009, Spain.ORCID 0000-0003-1081-8482
Valeria GrazúInstituto de Nanociencia y Materiales de Aragón, INMA (CSIC-Universidad de Zaragoza), C/Pedro Cerbuna 12, Zaragoza 50009, Spain.ORCID 0000-0001-6170-4237
Raluca M FratilaInstituto de Nanociencia y Materiales de Aragón, INMA (CSIC-Universidad de Zaragoza), C/Pedro Cerbuna 12, Zaragoza 50009, Spain.ORCID 0000-0001-5559-8757
María MorosInstituto de Nanociencia y Materiales de Aragón, INMA (CSIC-Universidad de Zaragoza), C/Pedro Cerbuna 12, Zaragoza 50009, Spain.ORCID 0000-0002-2861-2469
Universidad de Zaragoza · ESHospital General Universitario Gregorio Marañón · ES

Funding

European Research Council
6 · The paper itself

Abstract

Oriented and covalent immobilization of proteins on magnetic nanoparticles (MNPs) is particularly challenging as it requires both the functionality of the protein and the colloidal stability of the MNPs to be preserved. Here, we describe a simple, straightforward, and efficient strategy for MNP functionalization with proteins using metal affinity binding. Our method involves a single-step process where MNPs are functionalized using a preformed, ready-to-use nitrilotriacetic acid-divalent metal cation (NTA-M

Indexed as

Magnetite NanoparticlesCadherinsChelating AgentsIndicators and ReagentsMetalsNitrilotriacetic AcidCadherinsChelating AgentsIndicators and ReagentsMagnetite NanoparticlesMetalsNitrilotriacetic Acid

Identifiers

PMID37882455
PMCPMC10739578
OpenAlexW4387949379

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.