ArticlePlant biotechnology journal2024
A quantitative autonomous bioluminescence reporter system with a wide dynamic range for Plant Synthetic Biology.
Article in Plant biotechnology journal, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Expanding agroinfiltration host range with broad-spectrum effectors monitored by autoluminescent reporter.Horticulture research · 2026Article
- Designing and testing CRISPRi-based synthetic gene circuits in plants.Nature protocols · 2026Review
- Bioluminescent sentinel plants enable autonomous diagnostics of viral infections.Nature communications · 2026Article
- Remote Sensing of Endogenous Pigmentation by Inducible Synthetic Circuits in Grasses.Plant biotechnology journal · 2026Article
- Non-invasive imaging of defence responses in plants.Nature communications · 2026Article
- Sustainable bioenergy manufacturing in plants.Plant communications · 2026Review
- Advances in luminescence-based tracing technologies: applications and future perspectives.Biodesign research · 2026Review
- Engineering Conditional Transgene Expression in Nicotiana benthamiana.Plant biotechnology journal · 2026Review
- Cascade Oxygen Control Enhances Growth ofPlants (Basel, Switzerland) · 2025Article
- Bioluminescence-Driven Optimization of Geminivirus-Based Vectors as Tools for Plant Biotechnology.ACS synthetic biology · 2025Article
- The switch-liker's guide to plant synthetic gene circuits.The Plant journal : for cell and molecular biology · 2025Review
- Bacterial network for precise plant stress detection and enhanced crop resilience.BMC bioinformatics · 2025Article
- Illuminating the future: Enhanced glowing plants achieved by rewiring metabolism.The Plant cell · 2024Article
- Integration of biological and information technologies to enhance plant autoluminescence.The Plant cell · 2024Article
- Quantifying Plant Signaling Pathways by Integrating Luminescence-Based Biosensors and Mathematical Modeling.Biosensors · 2024Article
- An improved pathway for autonomous bioluminescence imaging in eukaryotes.Nature methods · 2024Article
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Authors and funding
4 authors.
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Abstract
Plant Synthetic Biology aims to enhance the capacities of plants by designing and integrating synthetic gene circuits (SGCs). Quantitative reporting solutions that can produce quick, rich datasets affordably are necessary for SGC optimization. In this paper, we present a new, low-cost, and high-throughput reporter system for the quantitative measurement of gene expression in plants based on autonomous bioluminescence. This method eliminates the need for an exogenous supply of luciferase substrate by exploiting the entire Neonothopanus nambi fungal bioluminescence cyclic pathway to build a self-sustained reporter. The HispS gene, the pathway's limiting step, was set up as the reporter's transcriptional entry point as part of the new system's design, which significantly improved the output's dynamic range and brought it on par with that of the gold standard FLuc/RLuc reporter. Additionally, transient ratiometric measurements in N. benthamiana were made possible by the addition of an enhanced GFP as a normalizer. The performance of new NeoLuc/eGFP system was extensively validated with SGCs previously described, including phytohormone and optogenetic sensors. Furthermore, we employed NeoLuc/eGFP in the optimization of challenging SGCs, including new configurations for an agrochemical (copper) switch, a new blue optogenetic sensor, and a dual copper/red-light switch for tight regulation of metabolic pathways.
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