ArticleFrontiers in cellular neuroscience2023
Fluorescence radial fluctuation enables two-photon super-resolution microscopy.
Article in Frontiers in cellular neuroscience, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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5 citing papers in PubMed, 10 citations in OpenAlex.
- Evaluating the applicability of computational super-resolution techniques for harmonic generation microscopy.Scientific reports · 2026Article
- Advances in multiscale myelin imaging: from classical histology to functional insights.Frontiers in cellular neuroscience · 2026Review
- Super-resolution algorithms for imaging FCS enhancement: A comparative study.Biophysical journal · 2025Article
- Minimally invasive, wide-field two-photon imaging of the brainstem at cellular resolution.Cell reports methods · 2025Article
- Fluorescence super-resolution microscopy via fluctuation-based multi-route synergy.Biomedical optics express · 2024Article
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4 authors at 3 institutions in 1 country.
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Abstract
Despite recent improvements in microscopy, it is still difficult to apply super-resolution microscopy for deep imaging due to the deterioration of light convergence properties in thick specimens. As a strategy to avoid such optical limitations for deep super-resolution imaging, we focused on super-resolution radial fluctuation (SRRF), a super-resolution technique based on image analysis. In this study, we applied SRRF to two-photon microscopy (2P-SRRF) and characterized its spatial resolution, suitability for deep observation, and morphological reproducibility in real brain tissue. By the comparison with structured illumination microscopy (SIM), it was confirmed that 2P-SRRF exhibited two-point resolution and morphological reproducibility comparable to that of SIM. The improvement in spatial resolution was also demonstrated at depths of more than several hundred micrometers in a brain-mimetic environment. After optimizing SRRF processing parameters, we successfully demonstrated
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