Evidence map›Paper›PMID 37880620›Full record

ArticleClinical proteomics2023

Change of histone H3 lysine 14 acetylation stoichiometry in human monocyte derived macrophages as determined by MS-based absolute targeted quantitative proteomic approach: HIV infection and methamphetamine exposure.

Katarzyna Macur, Andrew Schissel, Fang Yu, Shulei Lei, Brenda Morsey, Howard S Fox, Pawel Ciborowski

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In one paragraph

Article in Clinical proteomics, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed
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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Katarzyna MacurCore Facility Laboratories, Intercollegiate Faculty of Biotechnology UG & MUG, University of Gdańsk, Gdańsk, Poland. katarzyna.macur@ug.edu.pl.
Andrew SchisselDepartment of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE, USA.
Fang YuDepartment of Biostatistics, University of Nebraska Medical Center, Omaha, NE, USA.
Shulei LeiDepartment of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE, USA.
Brenda MorseyDepartment of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE, USA.
Howard S FoxDepartment of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE, USA.
Pawel CiborowskiDepartment of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE, USA. pciborowski@unmc.edu.

Funding

Therapeutics Core (Page 286)P30MH062261 · NIMH · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI FOX, HOWARD S · 2000 to 2021
$37.7M
Macrophage, Meth, HIV and Histones: An InterplayR01DA043258 · NIDA · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI CIBOROWSKI, PAWEL S, FOX, HOWARD S · 2016 to 2020
$3.1M
NIDA NIH HHS R01 DA043258NIH HHS R01 DA043258NIMH NIH HHS P30 MH062261
6 · The paper itself

Abstract

backgroundHistones posttranslational modification represent an epigenetic mechanism that regulate gene expression and other cellular processes. Quantitative mass spectrometry used for the absolute quantification of such modifications provides further insight into cellular responses to extracellular insults such as infections or toxins. Methamphetamine (Meth), a drug of abuse, is affecting the overall function of the immune system. In this report, we developed, validated and applied a targeted, MS-based quantification assay to measure changes in histone H3 lysine 14 acetylation (H3K14Ac) during exposure of human primary macrophages to HIV-1 infection and/or Meth.

methodsThe quantification assay was developed and validated to determine H3K14Ac stoichiometry in histones that were isolated from the nuclei of control (CIC) and exposed to Meth before (CIM) or/and after (MIM) HIV-infection human monocyte-derived macrophages (hMDM) of six donors. It was based on LC-MS/MS measurement using multiple reaction monitoring (MRM) acquisition of the unmodified and acetylated form of lysine K14 of histone H3

resultsThe assay was characterized by LLOD of 0.106 fmol/µL and 0.204 fmol/µL for unmodified and acetylated H3

conclusionsThe developed LC-MS/MS assay enabled absolute quantification of H3K14Ac in exposed to Meth HIV-infected hMDM. It can be further applied determination of this PTM stoichiometry in other studies on human primary macrophages.

Indexed as

Absolute quantificationHistone H3 lysine 14 acetylation (H3K14Ac)HIVLiquid chromatographyMass spectrometryMethamphetamineProteomics

Identifiers

PMID37880620
PMCPMC10599040

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