Evidence map›Paper›PMID 37879308›Full record

ArticleCytogenetic and genome research2023

Applicability of Scoring Calyculin A-Induced Premature Chromosome Condensation Objects for Dose Assessment Including for Radiotherapy Patients.

Mingzhu Sun, Jayne Moquet, David Lloyd, Stephen Barnard, Selvakumar Anbalagan, Harriet Steel, Aurore Sommer, Lone Gothard, Navita Somaiah, Elizabeth Ainsbury

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cytogenetic and genome research, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Mingzhu SunUK Health Security Agency (UKHSA), Department of Radiation Effects, Cytogenetics and Pathology Group, RCEHD, Didcot, UK.
Jayne MoquetUK Health Security Agency (UKHSA), Department of Radiation Effects, Cytogenetics and Pathology Group, RCEHD, Didcot, UK.
David LloydUK Health Security Agency (UKHSA), Department of Radiation Effects, Cytogenetics and Pathology Group, RCEHD, Didcot, UK.
Stephen BarnardUK Health Security Agency (UKHSA), Department of Radiation Effects, Cytogenetics and Pathology Group, RCEHD, Didcot, UK.
Selvakumar AnbalaganDivision of Radiotherapy and Imaging, The Institute of Cancer Research (ICR), Sutton, UK.
Harriet SteelDivision of Radiotherapy and Imaging, The Institute of Cancer Research (ICR), Sutton, UK.
Aurore SommerDivision of Radiotherapy and Imaging, The Institute of Cancer Research (ICR), Sutton, UK.
Lone GothardDivision of Radiotherapy and Imaging, The Institute of Cancer Research (ICR), Sutton, UK.
Navita SomaiahDivision of Radiotherapy and Imaging, The Institute of Cancer Research (ICR), Sutton, UK.
Elizabeth AinsburyUK Health Security Agency (UKHSA), Department of Radiation Effects, Cytogenetics and Pathology Group, RCEHD, Didcot, UK.
Institute of Cancer Research · GBRoyal Marsden NHS Foundation Trust · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

As an extension to a previous study, a linear calibration curve covering doses from 0 to 10 Gy was constructed and evaluated in the present study using calyculin A-induced premature chromosome condensation (PCC) by scoring excess PCC objects. The main aim of this study was to assess the applicability of this PCC assay for doses below 2 Gy that are critical for triage categorization. Two separate blind tests involving a total of 6 doses were carried out; 4 out of 6 dose estimates were within the 95% confidence limits (95% CL) with the other 2 just outside. In addition, blood samples from five cancer patients undergoing external beam radiotherapy (RT) were also analyzed, and the results showed whole-body dose estimates statistically comparable to the dicentric chromosome assay (DCA) results. This is the first time that calyculin A-induced PCC was used to analyze clinical samples by scoring excess objects. Although dose estimates for the pre-RT patient samples were found to be significantly higher than the mean value for the healthy donors and were also significantly higher than those obtained using DCA, all these pre-treatment patients fell into the same category as those who may have received a low dose (<1 Gy) and do not require immediate medical care during emergency triage. Additionally, for radiological accidents with unknown exposure scenario, PCC objects and rings can be scored in parallel for the assessment of both low- and high-dose exposures. In conclusion, scoring excess objects using calyculin A-induced PCC is confirmed to be another potential biodosimetry tool in radiological emergency particularly in mass casualty scenarios, even though the data need to be interpreted with caution when cancer patients are among the casualties.

Indexed as

LymphocytesNeoplasmsOxazolesChromosome AberrationsChromosomesHumansMarine ToxinsRadiometrycalyculin AMarine ToxinsOxazolesBiodosimetryNuclear emergencyPremature chromosome condensationRadiotherapy patient exposureTriage categorization

Identifiers

PMID37879308
PMCPMC10946622
OpenAlexW4387933594

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.