Evidence map›Paper›PMID 37878653›Full record

ArticlePloS one2023

Abdominal surgery induces long-lasting changes in expression and binding of CTCF with impact on Major Histocompatibility Complex II transcription in circulating human monocytes.

Benedikt Hermann Siegler, Jan Niklas Thon, Marc Altvater, Judith Schenz, Jan Larmann, Markus Alexander Weigand, Sebastian Weiterer

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.5field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Benedikt Hermann SieglerMedical Faculty Heidelberg, Department of Anesthesiology, Heidelberg University, Heidelberg, Baden-Württemberg, Germany.ORCID 0000-0002-9952-1564
Jan Niklas ThonMedical Faculty Heidelberg, Department of Anesthesiology, Heidelberg University, Heidelberg, Baden-Württemberg, Germany.
Marc AltvaterMedical Faculty Heidelberg, Department of Anesthesiology, Heidelberg University, Heidelberg, Baden-Württemberg, Germany.ORCID 0009-0009-2871-6856
Judith SchenzMedical Faculty Heidelberg, Department of Anesthesiology, Heidelberg University, Heidelberg, Baden-Württemberg, Germany.ORCID 0000-0002-6771-8864
Jan LarmannMedical Faculty Heidelberg, Department of Anesthesiology, Heidelberg University, Heidelberg, Baden-Württemberg, Germany.
Markus Alexander WeigandMedical Faculty Heidelberg, Department of Anesthesiology, Heidelberg University, Heidelberg, Baden-Württemberg, Germany.
Sebastian WeitererMedical Faculty Heidelberg, Department of Anesthesiology, Heidelberg University, Heidelberg, Baden-Württemberg, Germany.
Heidelberg University · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundPostoperative immunosuppression has been recognized as an important driver of surgery-related morbidity and mortality. It is characterized by lymphocyte depression and impaired monocyte capability to present foreign antigens to T-cells via Major Histocompatibility Complex, Class II (MHC-II) molecules. In patients with postoperative abdominal sepsis, we previously detected a persisting differential binding of the CCCTC-Binding Factor (CTCF), a superordinate regulator of transcription, inside the MHC-II region with specific impact on human leucocyte antigen (HLA) gene expression. In this prospective exploratory study, we investigated to which extent major surgery affects the MHC-II region of circulating CD14+-monocytes.

resultsIn non-immunocompromised patients undergoing elective major abdominal surgery, a postoperative loss of monocyte HLA-DR surface receptor density was accompanied by a decline in the transcription levels of the classical MHC-II genes HLA-DRA, HLA-DRB1, HLA-DPA1 and HLA-DPB1. The surgical event decreased the expression of the transcriptional MHC-II regulators CIITA and CTCF and led to a lower CTCF enrichment at an intergenic sequence within the HLA-DR subregion. During the observation period, we found a slow and only incomplete restoration of monocyte HLA-DR surface receptor density as well as a partial recovery of CIITA, HLA-DRA and HLA-DRB1 expression. In contrast, transcription of HLA-DPA1, HLA-DPB1, CTCF and binding of CTCF within the MHC-II remained altered.

conclusionIn circulating monocytes, major surgery does not globally affect MHC-II transcription but rather induces specific changes in the expression of selected HLA genes, followed by differential recovery patterns and accompanied by a prolonged reduction of CTCF expression and binding within the MHC-II region. Our results hint toward a long-lasting impact of a major surgical intervention on monocyte functionality, possibly mediated by epigenetic changes that endure the life span of the individual cell.

Indexed as

Gene Expression RegulationMonocytesCCCTC-Binding FactorGenes, MHC Class IIHistocompatibility Antigens Class IIHLA-DR alpha-ChainsHLA-DRB1 ChainsHumansProspective StudiesCCCTC-Binding FactorHistocompatibility Antigens Class IIHLA-DR alpha-ChainsHLA-DRB1 Chains

Identifiers

PMID37878653
PMCPMC10599505
OpenAlexW4387948411

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.