Evidence map›Paper›PMID 37877711›Full record

ArticleInfection and immunity2023

Reduction is sufficient for the disassembly of ricin and Shiga toxin 1 but not

Jessica L Guyette, Albert Serrano, G Robb Huhn Iii, Michael Taylor, Pat Malkòm, David Curtis, Ken Teter

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Infection and immunity, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Jessica L GuyetteBurnett School of Biomedical Sciences, College of Medicine, University of Central Florida , Orlando, Florida, USA.
Albert SerranoBurnett School of Biomedical Sciences, College of Medicine, University of Central Florida , Orlando, Florida, USA.
G Robb Huhn IiiBurnett School of Biomedical Sciences, College of Medicine, University of Central Florida , Orlando, Florida, USA.
Michael TaylorBurnett School of Biomedical Sciences, College of Medicine, University of Central Florida , Orlando, Florida, USA.
Pat MalkòmBurnett School of Biomedical Sciences, College of Medicine, University of Central Florida , Orlando, Florida, USA.
David CurtisBurnett School of Biomedical Sciences, College of Medicine, University of Central Florida , Orlando, Florida, USA.
Ken TeterBurnett School of Biomedical Sciences, College of Medicine, University of Central Florida , Orlando, Florida, USA.ORCID 0000-0003-4176-9361

Funding

The "Disaggregase" Mechanism of Holotoxin Disassembly by Protein Disulfide IsomeraseR01AI137056 · NIAID · UNIVERSITY OF CENTRAL FLORIDA · PI TETER, KENNETH R · 2018 to 2022
$2.0M
Toxin Potency is Linked to Holotoxin Disassembly by Protein Disulfide IsomeraseR03AI112854 · NIAID · UNIVERSITY OF CENTRAL FLORIDA · PI TETER, KENNETH R · 2015 to 2016
$146k
NIAID NIH HHS R01 AI137056NIAID NIH HHS R03 AI112854
6 · The paper itself

Abstract

Many AB toxins contain an enzymatic A moiety that is anchored to a cell-binding B moiety by a disulfide bridge. After receptor-mediated endocytosis, some AB toxins undergo retrograde transport to the endoplasmic reticulum (ER) where reduction of the disulfide bond occurs. The reduced A subunit then dissociates from the holotoxin and enters the cytosol to alter its cellular target. Intoxication requires A chain separation from the holotoxin, but, for many toxins, it is unclear if reduction alone is sufficient for toxin disassembly. Here, we examined the link between reduction and disassembly for several ER-translocating toxins. We found disassembly of the reduced

Indexed as

RicinBacterial ToxinsDisulfidesEnterotoxinsEscherichia coli ProteinsGlycosidesProtein Disulfide-IsomerasesShiga Toxin 1TriterpenesBacterial ToxinsDisulfidesEnterotoxinsEscherichia coli ProteinsGlycosidesheat-labile enterotoxin, E coliProtein Disulfide-IsomerasesRicinShiga Toxin 1stichoposideTriterpenesAB toxinERp57ERp72protein disulfide isomeraseShiga toxin 2surface plasmon resonance

Identifiers

PMID37877711
PMCPMC10652930

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.