Evidence map›Paper›PMID 37873360›Full record

ArticlebioRxiv : the preprint server for biology2023

A label-free approach for relative spatial quantitation of c-di-GMP in microbial biofilms.

Catherine S McCaughey, Michael A Trebino, Allyson McAtamney, Ruth Isenberg, Mark J Mandel, Fitnat H Yildiz, Laura M Sanchez

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Catherine S McCaugheyDepartment of Chemistry and Biochemistry, University of California, Santa Cruz, Santa Cruz, CA 95064.
Michael A TrebinoDepartment of Microbiology and Environmental Toxicology, University of California, Santa Cruz, Santa Cruz, CA 95064.
Allyson McAtamneyDepartment of Chemistry and Biochemistry, University of California, Santa Cruz, Santa Cruz, CA 95064.
Ruth IsenbergDepartment of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, WI 53706.
Mark J MandelDepartment of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, WI 53706.
Fitnat H YildizDepartment of Microbiology and Environmental Toxicology, University of California, Santa Cruz, Santa Cruz, CA 95064.
Laura M SanchezDepartment of Chemistry and Biochemistry, University of California, Santa Cruz, Santa Cruz, CA 95064.
University of California, Santa Cruz · USUniversity of Wisconsin–Madison · US

Funding

Vibrio cholerae c-diGMP signaling: Motile to biofilm transition and transmissionR01AI102584 · NIAID · UNIVERSITY OF CALIFORNIA SANTA CRUZ · PI YILDIZ, HAVVA FITNAT · 2013 to 2023
$6.5M
Genetic analysis of beneficial bacterial colonizationR35GM148385 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI Mark J Mandel · 2023 to 2026
$1.8M
NIAID NIH HHS R01 AI102584NIGMS NIH HHS R35 GM148385
6 · The paper itself

Abstract

Microbial biofilms represent an important lifestyle for bacteria and are dynamic three dimensional structures. Cyclic dimeric guanosine monophosphate (c-di-GMP) is a ubiquitous signaling molecule that is known to be tightly regulated with biofilm processes. While measurements of global levels of c-di-GMP have proven valuable towards understanding the genetic control of c-di-GMP production, there is a need for tools to observe the local changes of c-di-GMP production in biofilm processes. We have developed a label-free method for the direct detection of c-di-GMP in microbial colony biofilms using matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI). We applied this method to the enteric pathogen

Identifiers

PMID37873360
PMCPMC10592747
OpenAlexW4387536315

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.