Evidence map›Paper›PMID 37873314›Full record

ArticlebioRxiv : the preprint server for biology2023

Mapping of multiple neurotransmitter receptor subtypes and distinct protein complexes to the connectome.

Piero Sanfilippo, Alexander J Kim, Anuradha Bhukel, Juyoun Yoo, Pegah S Mirshahidi, Vijaya Pandey, Harry Bevir, Ashley Yuen, Parmis S Mirshahidi, Peiyi Guo and 4 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

14 authors at 3 institutions in 1 country.

Piero SanfilippoDepartment of Biological Chemistry, University of California Los Angeles, Los Angeles, CA, USA.
Alexander J KimDepartment of Biological Chemistry, University of California Los Angeles, Los Angeles, CA, USA.
Anuradha BhukelJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Juyoun YooNeuroscience Interdepartmental Program, University of California Los Angeles, Los Angeles, CA, USA.
Pegah S MirshahidiDepartment of Biological Chemistry, University of California Los Angeles, Los Angeles, CA, USA.
Vijaya PandeyDepartment of Biological Chemistry, University of California Los Angeles, Los Angeles, CA, USA.
Harry BevirDepartment of Biological Chemistry, University of California Los Angeles, Los Angeles, CA, USA.
Ashley YuenDepartment of Biological Chemistry, University of California Los Angeles, Los Angeles, CA, USA.
Parmis S MirshahidiDepartment of Biological Chemistry, University of California Los Angeles, Los Angeles, CA, USA.
Peiyi GuoDepartment of Neurobiology, University of Massachusetts Medical School, Worcester, MA, USA.
Hong-Sheng LiDepartment of Neurobiology, University of Massachusetts Medical School, Worcester, MA, USA.
James A WohlschlegelDepartment of Biological Chemistry, University of California Los Angeles, Los Angeles, CA, USA.
Yoshinori AsoJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
S Lawrence ZipurskyDepartment of Biological Chemistry, University of California Los Angeles, Los Angeles, CA, USA.
University of California, Los Angeles · USHoward Hughes Medical Institute · USUniversity of Massachusetts Chan Medical School · US

Funding

Proteolytic Control of Iron Metabolism by the Ubiquitin Ligase FBXL5R01GM089778 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI WOHLSCHLEGEL, JAMES AKIRA · 2010 to 2023
$4.0M
Mapping neurotransmitter receptors onto the connectomeRF1MH117823 · NIMH · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ZIPURSKY, S. LAWRENCE · 2018 to 2018
$1.2M
NIGMS NIH HHS R01 GM089778NIMH NIH HHS RF1 MH117823
6 · The paper itself

Abstract

Neurons express different combinations of neurotransmitter receptor (NR) subunits and receive inputs from multiple neuron types expressing different neurotransmitters. Localizing NR subunits to specific synaptic inputs has been challenging. Here we use epitope tagged endogenous NR subunits, expansion light-sheet microscopy, and EM connectomics to molecularly characterize synapses in Drosophila. We show that in directionally selective motion sensitive neurons, different multiple NRs elaborated a highly stereotyped molecular topography with NR localized to specific domains receiving cell-type specific inputs. Developmental studies suggested that NRs or complexes of them with other membrane proteins determines patterns of synaptic inputs. In support of this model, we identify a transmembrane protein associated selectively with a subset of spatially restricted synapses and demonstrate through genetic analysis its requirement for synapse formation. We propose that mechanisms which regulate the precise spatial distribution of NRs provide a molecular cartography specifying the patterns of synaptic connections onto dendrites.

Identifiers

PMID37873314
PMCPMC10592863
OpenAlexW4387244251

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.