Evidence map›Paper›PMID 37873297›Full record

ArticlebioRxiv : the preprint server for biology2023

Rtf1-dependent transcriptional pausing regulates cardiogenesis.

Adam D Langenbacher, Fei Lu, Luna Tsang, Zi Yi Stephanie Huang, Benjamin Keer, Zhiyu Tian, Alette Eide, Matteo Pellegrini, Haruko Nakano, Atsushi Nakano and 1 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 3 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors at 1 institution in 1 country.

Adam D LangenbacherDepartment of Molecular, Cell, and Developmental Biology, University of California Los Angeles, Los Angeles, California, United States of America.
Fei LuDepartment of Molecular, Cell, and Developmental Biology, University of California Los Angeles, Los Angeles, California, United States of America.
Luna TsangDepartment of Molecular, Cell, and Developmental Biology, University of California Los Angeles, Los Angeles, California, United States of America.
Zi Yi Stephanie HuangDepartment of Molecular, Cell, and Developmental Biology, University of California Los Angeles, Los Angeles, California, United States of America.
Benjamin KeerDepartment of Molecular, Cell, and Developmental Biology, University of California Los Angeles, Los Angeles, California, United States of America.
Zhiyu TianDepartment of Molecular, Cell, and Developmental Biology, University of California Los Angeles, Los Angeles, California, United States of America.
Alette EideDepartment of Molecular, Cell, and Developmental Biology, University of California Los Angeles, Los Angeles, California, United States of America.
Matteo PellegriniDepartment of Molecular, Cell, and Developmental Biology, University of California Los Angeles, Los Angeles, California, United States of America.
Haruko NakanoDepartment of Molecular, Cell, and Developmental Biology, University of California Los Angeles, Los Angeles, California, United States of America.
Atsushi NakanoDepartment of Molecular, Cell, and Developmental Biology, University of California Los Angeles, Los Angeles, California, United States of America.
Jau-Nian ChenDepartment of Molecular, Cell, and Developmental Biology, University of California Los Angeles, Los Angeles, California, United States of America.
University of California, Los Angeles · US

Funding

Rtf1-dependent transcriptional regulation of heart developmentR01HL140472 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI CHEN, JAU-NIAN, TRINH, LE A. · 2018 to 2021
$1.6M
Impacts of transcription elongation on cardiac gene regulation during homeostasis and regenerationR01HL155905 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI CHEN, JAU-NIAN · 2021 to 2024
$1.6M
NHLBI NIH HHS R01 HL140472NHLBI NIH HHS R01 HL155905
6 · The paper itself

Abstract

During heart development, a well-characterized network of transcription factors initiates cardiac gene expression and defines the precise timing and location of cardiac progenitor specification. However, our understanding of the post-initiation transcriptional events that regulate cardiac gene expression is still incomplete. The PAF1C component Rtf1 is a transcription regulatory protein that modulates pausing and elongation of RNA Pol II, as well as cotranscriptional histone modifications. Here we report that Rtf1 is essential for cardiogenesis in fish and mammals, and that in the absence of Rtf1 activity, cardiac progenitors arrest in an immature state. We found that Rtf1's Plus3 domain, which confers interaction with the transcriptional pausing and elongation regulator Spt5, was necessary for cardiac progenitor formation. ChIP-seq analysis further revealed changes in the occupancy of RNA Pol II around the transcription start site (TSS) of cardiac genes in

Indexed as

cardiogenesislateral plate mesodermPAF1CPAF1 complexpromoter-proximal pausingRtf1transcription regulation

Identifiers

PMID37873297
PMCPMC10592831
OpenAlexW4387652168

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.