Evidence map›Paper›PMID 37873262›Full record

ArticlebioRxiv : the preprint server for biology2023

Light-induced trapping of endogenous proteins reveals spatiotemporal roles of microtubule and kinesin-1 in dendrite patterning of

Yineng Xu, Bei Wang, Inle Bush, Harriet Aj Saunders, Jill Wildonger, Chun Han

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Yineng XuWeill Institute for Cell and Molecular Biology, Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA.
Bei WangWeill Institute for Cell and Molecular Biology, Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA.
Inle BushWeill Institute for Cell and Molecular Biology, Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA.
Harriet Aj SaundersDepartment of Biochemistry, University of Wisconsin-Madison, 440 Henry Mall, Madison, WI 53706, USA.
Jill WildongerDepartment of Biochemistry, University of Wisconsin-Madison, 440 Henry Mall, Madison, WI 53706, USA.
Chun HanWeill Institute for Cell and Molecular Biology, Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA.
Cornell University · USUniversity of Wisconsin–Madison · US

Funding

MAGIC tools for genome-wide mosaic analysis with existing Drosophila resources (Equipment Supplement 2023)R24OD031953 · OD · CORNELL UNIVERSITY · PI HAN, CHUN · 2022 to 2025
$3.1M
Mechanisms of the recognition of degenerating dendritesR01NS099125 · NINDS · CORNELL UNIVERSITY · PI HAN, CHUN · 2016 to 2020
$2.1M
MOLECULAR MOTORS AND NEURONAL MICROTUBULE POLARITYR01NS102385 · NINDS · UNIVERSITY OF WISCONSIN-MADISON · PI WILDONGER, JILL C · 2017 to 2022
$1.6M
A light-inducible protein trapping system for studying cellular dynamics in DrosophilaR21OD023824 · OD · CORNELL UNIVERSITY · PI HAN, CHUN · 2017 to 2018
$414k
NIH HHS R21 OD023824NIH HHS R24 OD031953NINDS NIH HHS R01 NS099125NINDS NIH HHS R01 NS102385
6 · The paper itself

Abstract

Animal development involves numerous molecular events, whose spatiotemporal properties largely determine the biological outcomes. Conventional methods for studying gene function lack the necessary spatiotemporal resolution for precise dissection of developmental mechanisms. Optogenetic approaches are powerful alternatives, but most existing tools rely on exogenous designer proteins that produce narrow outputs and cannot be applied to diverse or endogenous proteins. To address this limitation, we developed OptoTrap, a light-inducible protein trapping system that allows manipulation of endogenous proteins tagged with GFP or split GFP. This system turns on fast and is reversible in minutes or hours. We generated OptoTrap variants optimized for neurons and epithelial cells and demonstrate effective trapping of endogenous proteins of diverse sizes, subcellular locations, and functions. Furthermore, OptoTrap allowed us to instantly disrupt microtubules and inhibit the kinesin-1 motor in specific dendritic branches of

Indexed as

CRY2oligdendritedendritic arborization neuronDrosophilakinesinmicrotubulenMagOptogeneticsOptoTrappMagprotein trapping

Identifiers

PMID37873262
PMCPMC10592855
OpenAlexW4387246401

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.