Evidence map›Paper›PMID 37873121›Full record

ArticlebioRxiv : the preprint server for biology2023

Activation of automethylated PRC2 by dimerization on chromatin.

Paul V Sauer, Egor Pavlenko, Trinity Cookis, Linda C Zirden, Juliane Renn, Ankush Singhal, Pascal Hunold, Michaela N Hoehne, Olivia van Ray, Robert Hänsel-Hertsch and 3 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 3 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

13 authors at 4 institutions in 2 countries.

Paul V SauerCalifornia Institute for Quantitative Biology (QB3), University of California, Berkeley, California 94720, USA.
Egor PavlenkoCenter for Molecular Medicine Cologne (CMMC), Faculty of Medicine and University Hospital, University of Cologne, Germany.
Trinity CookisDepartment of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA.
Linda C ZirdenCenter for Molecular Medicine Cologne (CMMC), Faculty of Medicine and University Hospital, University of Cologne, Germany.
Juliane RennCenter for Molecular Medicine Cologne (CMMC), Faculty of Medicine and University Hospital, University of Cologne, Germany.
Ankush SinghalTheoretical Biology and Biophysics, Theoretical Division, Los Alamos National Laboratory.
Pascal HunoldCenter for Molecular Medicine Cologne (CMMC), Faculty of Medicine and University Hospital, University of Cologne, Germany.
Michaela N HoehneCenter for Molecular Medicine Cologne (CMMC), Faculty of Medicine and University Hospital, University of Cologne, Germany.
Olivia van RayCenter for Molecular Medicine Cologne (CMMC), Faculty of Medicine and University Hospital, University of Cologne, Germany.
Robert Hänsel-HertschCenter for Molecular Medicine Cologne (CMMC), Faculty of Medicine and University Hospital, University of Cologne, Germany.
Karissa Y SanbonmatsuTheoretical Biology and Biophysics, Theoretical Division, Los Alamos National Laboratory.
Eva NogalesCalifornia Institute for Quantitative Biology (QB3), University of California, Berkeley, California 94720, USA.
Simon PoepselCenter for Molecular Medicine Cologne (CMMC), Faculty of Medicine and University Hospital, University of Cologne, Germany.
University of Cologne · DELos Alamos National Laboratory · USQB3 · USUniversity of California, Berkeley · US

Funding

ChimeraX -- Next Generation Visualization and Analysis Software for Multiscale ModelingR01GM129325 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI FERRIN, THOMAS E · 2018 to 2025
$5.2M
Structural studies of function and regulation of microtubules and transcriptional gene expression machineryR35GM127018 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI Eva Nogales · 2018 to 2026
$4.3M
NIGMS NIH HHS R01 GM129325NIGMS NIH HHS R35 GM127018
6 · The paper itself

Abstract

Polycomb Repressive Complex 2 (PRC2) is an epigenetic regulator that trimethylates lysine 27 of histone 3 (H3K27me3) and is essential for embryonic development and cellular differentiation. H3K27me3 is associated with transcriptionally repressed chromatin and is established when PRC2 is allosterically activated upon methyl-lysine binding by the regulatory subunit EED. Automethylation of the catalytic subunit EZH2 stimulates its activity by an unknown mechanism. Here, we show that PRC2 forms a dimer on chromatin in which an inactive, automethylated PRC2 protomer is the allosteric activator of a second PRC2 that is poised to methylate H3 of a substrate nucleosome. Functional assays support our model of allosteric

Indexed as

chromatincryo-EMdevelopmentepigeneticsgene regulation

Identifiers

PMID37873121
PMCPMC10592840
OpenAlexW4387641515

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.