ArticleArchives of gynecology and obstetrics2024
A method for isolating and culturing ectopic epithelial and stromal cells to study human adenomyosis.
Article in Archives of gynecology and obstetrics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed, 3 citations in OpenAlex.
- Improved surgical outcomes in adenomyosis: a novel insight into the superiority of secretory phase adenomyomectomy.Archives of gynecology and obstetrics · 2025Article
- Primary culture of endometrial mesenchymal stem cells derived from ectopic lesions of patients with adenomyosis.Archives of gynecology and obstetrics · 2024Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
purposeAlthough adenomyosis is a common and benign gynecological disease, the specific pathogenesis of this condition is yet to be fully elucidated. It is difficult to culture primary cells of the ectopic endometrial epithelia and stroma from human adenomyosis lesions. Most of the previous of studies on adenomyosis were based on primary eutopic endometrium cells. However, as yet, no efficient protocols have been developed for the isolation, culture or purification of primary ectopic epithelial and stromal cells from human adenomyosis lesions. Therefore, the present study aimed to develop an efficient protocol for the isolation and culture of primary ectopic epithelial and stromal cells from human adenomyosis lesions.
methodsIn the present study, we aimed to obtain ectopic endometrium tissue from human adenomyosis foci and use a simple and operable type I collagenase digestion method for primary culture. Cells were isolated by sterile cell strainer filtration and flow cytometry was performed to identify, purify, and evaluate the viability of isolated ectopic endometrial cells.
resultsUsing our method, we successfully isolated and cultured highly purified and active ectopic endometrial epithelial and stromal cells from human adenomyosis foci. Ep-CAM was expressed in ectopic epithelial cells of human adenomyosis with a purity of 93.74% and a viability of 80.58%. In addition, CD10 were robustly expressed by ectopic stromal cells in human adenomyosis. Cellular purity and viability were determined to be 96.37 and 93.49%, respectively.
conclusionOur method provides a new experimental model for studying the molecular pathogenesis of human adenomyosis.
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