Evidence map›Paper›PMID 37870741›Full record

ArticleMarine biotechnology (New York, N.Y.)2023

DNA Methylation Profiling of Ovarian Tissue of Climbing Perch (Anabas testudienus) in Response to Monocrotophos Exposure.

Kiran D Rasal, Sujata Mohapatra, Pokanti Vinay Kumar, Shasti Risha K, Prachi Asgolkar, Arpit Acharya, Diganta Dey, Siba Shinde, Manohar Vasam, Rajesh Kumar and 1 more

Abstract read
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Article in Marine biotechnology (New York, N.Y.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.8field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Kiran D RasalICAR-Central Institute of Freshwater Aquaculture, Bhubaneswar, 751 002, Odisha, India.
Sujata MohapatraICAR-Central Institute of Freshwater Aquaculture, Bhubaneswar, 751 002, Odisha, India.
Pokanti Vinay KumarICAR-Central Institute of Fisheries Education, Mumbai, 400 061, Maharashtra, India.
Shasti Risha KICAR-Central Institute of Fisheries Education, Mumbai, 400 061, Maharashtra, India.
Prachi AsgolkarICAR-Central Institute of Fisheries Education, Mumbai, 400 061, Maharashtra, India.
Arpit AcharyaICAR-Central Institute of Fisheries Education, Mumbai, 400 061, Maharashtra, India.
Diganta DeyICAR-Central Institute of Fisheries Education, Mumbai, 400 061, Maharashtra, India.
Siba ShindeICAR-Central Institute of Fisheries Education, Mumbai, 400 061, Maharashtra, India.
Manohar VasamICAR-Central Institute of Freshwater Aquaculture, Bhubaneswar, 751 002, Odisha, India.
Rajesh KumarICAR-Central Institute of Freshwater Aquaculture, Bhubaneswar, 751 002, Odisha, India.
Jitendra Kumar SundarayICAR-Central Institute of Freshwater Aquaculture, Bhubaneswar, 751 002, Odisha, India. jsundaray@gmail.com.
Central Institute of Fisheries Education · INCentral Institute of Freshwater Aquaculture · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Epigenetic modifications like DNA methylation can alter an organism's phenotype without changing its DNA sequence. Exposure to environmental toxicants has the potential to change the resilience of aquatic species. However, little information is available on the dynamics of DNA methylation in fish gonadal tissues in response to organophosphates. In the present work, reduced-representation bisulfite sequencing was performed to identify DNA methylation patterns in the ovarian tissues of Anabas testudienus exposed to organophosphates, specifically monocrotophos (MCP). Through sequencing, an average of 41,087 methylated cytosine sites were identified and distributed in different parts of genes, i.e., in transcription start sites (TSS), promoters, exons, etc. A total of 1058 and 1329 differentially methylated regions (DMRs) were detected as hyper-methylated and hypo-methylated in ovarian tissues, respectively. Utilizing whole-genome data of the climbing perch, the DMRs, and their associated overlapping genes revealed a total of 22 genes within exons, 45 genes at transcription start sites (TSS), and 218 genes in intergenic regions. Through gene ontology analysis, a total of 16 GO terms particularly involved in ovarian follicular development, response to oxidative stress, oocyte maturation, and multicellular organismal response to stress associated with reproductive biology were identified. After functional enrichment analysis, relevant DMGs such as steroid hormone biosynthesis (Cyp19a, 11-beta-HSD, 17-beta-HSD), hormone receptors (ar, esrrga), steroid metabolism (StAR), progesterone-mediated oocyte maturation (igf1ar, pgr), associated with ovarian development in climbing perch showed significant differential methylation patterns. The differentially methylated genes (DMGs) were subjected to analysis using real-time PCR, which demonstrated altered gene expression levels. This study revealed a molecular-level alteration in genes associated with ovarian development in response to chemical exposure. This work provides evidence for understanding the relationship between DNA methylation and gene regulation in response to chemicals that affect the reproductive fitness of aquatic animals.

Indexed as

MonocrotophosPerchesAnimalsDNA MethylationEpigenesis, GeneticHormonesSteroidsHormonesMonocrotophosSteroidsAnabas testudineusDNA methylationMonocrotophosNext generation sequencingReduced-representation bisulfite sequencing

Identifiers

PMID37870741
OpenAlexW4387865264

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.