Evidence map›Paper›PMID 37868356›Full record

ArticleACS measurement science au2023

Accessible and Generalizable in Vitro Luminescence Assay for Detecting GPCR Activation.

Ruby M Miller, Jennifer Sescil, Marina C Sarcinella, Ryan C Bailey, Wenjing Wang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS measurement science au, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.2field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Ruby M MillerLife Sciences Institute, University of Michigan, Ann Arbor, Michigan 48109, United States.
Jennifer SescilLife Sciences Institute, University of Michigan, Ann Arbor, Michigan 48109, United States.
Marina C SarcinellaDepartment of Chemistry, University of Michigan, Ann Arbor, Michigan 48109, United States.
Ryan C BaileyDepartment of Chemistry, University of Michigan, Ann Arbor, Michigan 48109, United States.ORCID https://orcid.org/0000-0003-1021-4267
Wenjing WangLife Sciences Institute, University of Michigan, Ann Arbor, Michigan 48109, United States.ORCID https://orcid.org/0000-0001-6025-9848
University of Michigan · US

Funding

New classes of optogenetic and chemogenetic tools with a feedback controlDP2MH132939 · NIMH · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI WANG, WENJING · 2022 to 2025
$2.3M
GPCR activation-based in vitro luminescent assays for high-throughput drug screening and extracted GPCR characterizationF31DA056192 · NIDA · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI MILLER, RUBY MAY · 2022 to 2023
$55k
NIDA NIH HHS F31 DA056192NIMH NIH HHS DP2 MH132939
6 · The paper itself

Abstract

G protein-coupled receptors (GPCRs) serve critical physiological roles as the most abundant family of receptors. Here, we describe the design of a generalizable and cell lysate-based method that leverages the interaction between an agonist-activated GPCR and a conformation-specific binder to reconstitute split nanoluciferase (NanoLuc) in vitro. This tool,

Identifiers

PMID37868356
PMCPMC10588934
OpenAlexW4383498233

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.