Evidence map›Paper›PMID 37865878›Full record

ArticleCell journal2023

Unraveling The Effects of DICER1 Overexpression on Immune-Related Genes Expression in Mesenchymal Stromal/Stem Cells: Insights for Therapeutic Applications.

Hamid Reza Bidkhori, Moein Farshchian, Halimeh Hasanzadeh, Reza Jafarzadeh Esfehani, Reihaneh Alsadat Mahmoudian, Mahdi Moradi Marjaneh, Houshang Rafatpanah

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell journal, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 50% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 0 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 3 countries.

Hamid Reza BidkhoriImmunology Research Centre, Division of Inflammation and Inflammatory Diseases, Mashhad University of Medical Sciences, Mashhad, Iran.ORCID 0000-0002-0204-0351
Moein FarshchianStem Cells and Regenerative Medicine Department, Academic Center for Education, Culture, and Research (ACECR) - Khorasan Razavi, Iran.ORCID 0000-0002-1228-048X
Halimeh HasanzadehStem Cells and Regenerative Medicine Department, Academic Center for Education, Culture, and Research (ACECR) - Khorasan Razavi, Iran.ORCID 0000-0003-3694-4954
Reza Jafarzadeh EsfehaniBlood Borne Infections Research Center, Academic Center for Education, Culture, and Research (ACECR) - Khorasan Razavi, Iran.
Reihaneh Alsadat MahmoudianBasic Sciences Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran.ORCID 0000-0002-0003-9015
Mahdi Moradi MarjanehDepartment of Infectious Disease, Faculty of Medicine, Imperial College London, London, United Kingdom.ORCID 0000-0002-9412-9029
Houshang RafatpanahImmunology Research Centre, Division of Inflammation and Inflammatory Diseases, Mashhad University of Medical Sciences, Mashhad, Iran. Email: RafatPanahH@mums.ac.ir.ORCID 0000-0002-1061-5864
Mashhad University of Medical Sciences · IRImperial College London · GBUniversity of Modena and Reggio Emilia · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveThe immunoregulatory properties of mesenchymal stromal/stem cells (MSCs) bring a promise for the treatment of inflammatory diseases. However, their ability to suppress the immune system is unstable. To enhance their effectiveness against immune responses, it may be necessary to manipulate MSCs. Although some dsRNA transcripts come from invading viruses, the majority of dsRNA has an endogenous origin and is known as endo-siRNA. DICER1 is a ribonuclease protein that can generate small RNAs to modulate gene expression at the post-transcriptional level. We aimed to evaluate the expression of several immune-related genes at mRNA and protein levels in MSCs overexpressing DICER1 exogenously. MATERIALS AND

methodsIn this comparative transcriptomic experimental study, the adipose-derived MSCs (Ad-MSCs) were transfected using the pCAGGS-Flag-hsDicer vector for the

resultsThe overexpression of

conclusionIt seems that the ectopic expression of DICER1 in Ad-MSCs is linked to alterations in the expression level of immune-related genes. It is suggested that the manipulation of immune-related pathways in MSCs via the Dicer1 overexpression could facilitate the development of MSCs with distinct immunoregulatory phenotypes.

Indexed as

DICER1ImmunomodulationMesenchymal Stromal/Stem CellsRNA-sequencing

Identifiers

PMID37865878
PMCPMC10591266
OpenAlexW4387855427

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.