Evidence map›Paper›PMID 37864661›Full record

ArticleMolecular biology reports2023

The toxic profile of tramadol combined with nicotine on the liver and testicles: evidence from endoplasmic reticulum stress.

Doaa Ghorab, Ejlal M Abu-El-Rub, Mohamed Hamdi Gharaibeh, Ramada R Khasawneh, Rawan A Almazari, Ahmed Al-Emam, Ahmed Mohamed Helaly

Abstract read
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Article in Molecular biology reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 69% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 3 countries.

Doaa GhorabBasic Sciences Department, Faculty of Medicine, Yarmouk University, Irbid, Jordan.
Ejlal M Abu-El-RubBasic Sciences Department, Faculty of Medicine, Yarmouk University, Irbid, Jordan.
Mohamed Hamdi GharaibehBasic Veterinary Department, Faculty of Veterinary Medicine, Jordan University of Science and Technology, Ar-Ramtha, Jordan.
Ramada R KhasawnehBasic Sciences Department, Faculty of Medicine, Yarmouk University, Irbid, Jordan.
Rawan A AlmazariBasic Sciences Department, Faculty of Medicine, Yarmouk University, Irbid, Jordan.
Ahmed Al-EmamPathology Department, Medical School, King Khaled University, Abha, Kingdom of Saudi Arabia.
Ahmed Mohamed HelalyClinical Sciences Department, Faculty of Medicine, Yarmouk University, Irbid, Jordan. ahmedhelaly@mans.edu.eg.ORCID http://orcid.org/0000-0003-2510-7229
Mansoura University · EGYarmouk University · JOJordan University of Science and Technology · JO

Funding

Yarmouk University 11/2021
6 · The paper itself

Abstract

backgroundTramadol is one of the most commonly abused substances in the Middle East. Furthermore, smoking is extremely common among the population.

methodsAn experimental study was performed on Sprague-Dawley rats to explore the effects of both nicotine and tramadol on the liver and testes. The tramadol was administered at 10 and 20 mg/kg, respectively, while the nicotine was administered at 125 mg/kg. Histological examination and androgen receptor ELISA assay showed mild effects on the liver and proofed safety on the testis. Western blot analysis of BIP (immunoglobulin heavy-chain binding protein) and CHOP (CCAAT-enhancer-binding protein homologous protein) revealed that fewer problems were induced by adding nicotine to tramadol. Autophagy marker LCIII and apoptosis marker caspase-8 showed similar effects to CHOP and BIP on liver samples. The real-time PCR of BIP expression showed similar but not identical results.

conclusionsThe results showed mild endoplasmic reticulum stress, autophagy, and apoptosis in the liver samples. Histological examination revealed stable spermatogenesis with average androgen receptor blood levels in the different groups.

Indexed as

TestisTramadolAnimalsApoptosisEndoplasmic Reticulum Chaperone BiPEndoplasmic Reticulum StressLiverMaleNicotineRatsRats, Sprague-DawleyReceptors, AndrogenEndoplasmic Reticulum Chaperone BiPNicotineReceptors, AndrogenTramadolBIBCHOPEndoplasmic reticulum stressLiverNicotineTestisTramadol

Identifiers

PMID37864661
OpenAlexW4387848202

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.