Evidence map›Paper›PMID 37864529›Full record

ArticleAlcohol, clinical & experimental research2023

Alcohol use disorder (AUD) is associated with enhanced sensitivity to cellular lipopolysaccharide challenge.

Elizabeth M Burnette, Erica N Grodin, Richard Olmstead, Lara A Ray, Michael R Irwin

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Alcohol, clinical & experimental research, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.3field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 5 citations in OpenAlex.

  1. Trial
  2. Review
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Elizabeth M BurnetteDepartment of Psychology, University of California at Los Angeles, Los Angeles, California, USA.ORCID https://orcid.org/0000-0003-4005-5885
Erica N GrodinDepartment of Psychology, University of California at Los Angeles, Los Angeles, California, USA.ORCID https://orcid.org/0000-0001-5528-4918
Richard OlmsteadDepartment of Psychiatry and Biobehavioral Sciences, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, California, USA.
Lara A RayDepartment of Psychology, University of California at Los Angeles, Los Angeles, California, USA.
Michael R IrwinDepartment of Psychology, University of California at Los Angeles, Los Angeles, California, USA.
University of California, Los Angeles · USUniversity of Southern California · US

Funding

UCLA Training Program in Translational Neuroscience of Drug Abuse (TNDA)T32DA024635 · NIDA · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI LARA A. RAY, Kate M Wassum · 2008 to 2026
$6.2M
Alcoholism: Sleep and Cytokines in African AmericansR01AA013239 · NIAAA · VETERANS MEDICAL RESEARCH FDN/SAN DIEGO · PI IRWIN, MICHAEL R · 2001 to 2005
$1.9M
Clinical Neuroscience of Alcoholism: Integrating Neuroscience and Clinical TrialsK24AA025704 · NIAAA · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI LARA A. RAY · 2018 to 2026
$1.3M
Characterizing the Microbiome-Gut-Brain Axis in Individuals with Alcohol Use DisorderK01AA029712 · NIAAA · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Erica N Grodin · 2022 to 2026
$819k
Probing Inflammation and Reward Sensitivity in Alcohol Use DisorderF31AA028976 · NIAAA · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI BURNETTE, ELIZABETH · 2020 to 2022
$103k
NIAAA NIH HHS F31 AA028976NIAAA NIH HHS F31AA028976NIAAA NIH HHS K01 AA029712NIAAA NIH HHS K01AA029712NIAAA NIH HHS K24 AA025704NIAAA NIH HHS K24AA025704NIAAA NIH HHS R01 AA013239NIAAA NIH HHS R01AA13239NIDA NIH HHS T32 DA024635
6 · The paper itself

Abstract

backgroundInflammation has been associated with alcohol use disorder (AUD). A novel method to characterize AUD-related immune signaling involves probing Toll-like receptor (TLR)-4 stimulated monocyte production of intracellular cytokines (ICCs) via lipopolysaccharide (LPS). We evaluated relationships between AUD and ICC production at rest and after LPS stimulation.

methodsWe analyzed blood samples from 36 participants (AUD N = 14; Controls N = 22), collected across time, with ICC expression assessed at rest (i.e., unstimulated) and following stimulation with LPS (i.e., a total of 5 repeated unstimulated or stimulated measures/participant). Markers assessed included tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), TNF-α and IL-6 co-expression, and interferon (IFN). For each marker, we constructed linear mixed models with AUD, LPS, and timepoint as fixed effects (BMI as covariate), allowing for random slope and intercept. AUD × LPS was included as an interaction.

resultsFor TLR4-stimulated monocyte production of TNF-α, there were effects of AUD (p < 0.01), LPS (p < 0.001), and AUD × LPS interaction (p < 0.05), indicating that individuals with AUD showed greater unstimulated- and stimulated monocyte expression of TNF-α. Similarly, for TLR4-stimulated monocyte co-expression of TNF-α and IL-6, there were effects of AUD (p < 0.01), LPS (p < 0.001), and AUD × LPS interaction (p < 0.05). No AUD or LPS effects were found for IL-6. Timepoint effects were observed on IL-6 and TNF-α/IL-6 co-expression (p < 0.001). Finally, for IFN there were also effects of AUD (p < 0.05), LPS (p < 0.001), and AUD × LPS (p < 0.001).

conclusionsIndividuals with AUD showed greater resting or unstimulated levels of intracellular monocyte expression of TNF-α and IL-6/TNF-α co-expression than controls. AUD was associated with increases in TLR4-stimulated monocyte production of TNF-α and co-production of IL-6 and TNF-α. This is, to our knowledge, the first study to investigate relationships between AUD and monocyte production of proinflammatory cytokines, at rest and in response to TLR4 stimulation with LPS. The study extends previous findings on the roles of proinflammatory cytokines in AUD and serves as a critical proof of concept for the use of this method to probe neuroimmune mechanisms underlying AUD.

Indexed as

alcohol use disordercytokineinflammationlipopolysaccharide

Identifiers

PMID37864529
PMCPMC10830126
OpenAlexW4386166581

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.