ArticleNature communications2023
Insights into the missing apiosylation step in flavonoid apiosides biosynthesis of Leguminosae plants.
Article in Nature communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed.
- Structural insights into pterocarpan reductases unveil a universal ring-opening mechanism in plant biosynthesis of 4-(furan-2-yl) phenol derivatives.Acta pharmaceutica Sinica. B · 2026Article
- Convergent Evolution and De Novo Reconstitution of Apiin Biosynthesis.Plant biotechnology journal · 2026Article
- Valorization of ligustrum species: biosynthesis, metabolic engineering, and pharmacology of bioactive compounds.Bioresources and bioprocessing · 2026Review
- Advances in understanding the functions and regulatory factors of secondary metabolites inPlant signaling & behavior · 2025Review
- Apiin Promotes Healthy Aging inInternational journal of molecular sciences · 2025Article
- Comprehensive engineering of novel glycosyltransferase for efficient, donor-promiscuous, and regioselective glycosylation of flavonoids.Science advances · 2025Article
- Glycyrrhiza, a commonly used medicinal herb: Review of species classification, pharmacology, active ingredient biosynthesis, and synthetic biology.Journal of advanced research · 2025Review
- A simple widely applicable hairy root transformation method for gene function studies in medicinal plants.Acta pharmaceutica Sinica. B · 2025Article
- Molecular characterization and structural basis of a promiscuous glycosyltransferase for β-(1,6) oligoglucoside chain glycosides biosynthesis.Plant biotechnology journal · 2025Article
- Elucidation of the biosynthetic pathway of hydroxysafflor yellow A.Nature communications · 2025Article
- Structural Insights into the Substrate Recognition of Ginsenoside Glycosyltransferase Pq3-O-UGT2.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Estimation of the heritability of medicinal compound contents in Glycyrrhiza uralensis.PloS one · 2025Article
- Computational-guided discovery of UDP-glycosyltransferases for lauryl glucoside production using engineered E. coli.Bioresources and bioprocessing · 2024Article
- Functional characterization, structural basis, and protein engineering of a rare flavonoid 2'-Acta pharmaceutica Sinica. B · 2024Article
- Functional Identification ofInternational journal of molecular sciences · 2024Article
- Design and optimization of heat pump with infrared drying forFrontiers in nutrition · 2024Article
- The sugar donor specificity of plant family 1 glycosyltransferases.Frontiers in bioengineering and biotechnology · 2024Review
- Biochemical Characterization of Parsley Glycosyltransferases Involved in the Biosynthesis of a Flavonoid Glycoside, Apiin.International journal of molecular sciences · 2023Article
- Structure-function and engineering of plant UDP-glycosyltransferase.Computational and structural biotechnology journal · 2023Review
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12 authors.
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Abstract
Apiose is a natural pentose containing an unusual branched-chain structure. Apiosides are bioactive natural products widely present in the plant kingdom. However, little is known on the key apiosylation reaction in the biosynthetic pathways of apiosides. In this work, we discover an apiosyltransferase GuApiGT from Glycyrrhiza uralensis. GuApiGT could efficiently catalyze 2″-O-apiosylation of flavonoid glycosides, and exhibits strict selectivity towards UDP-apiose. We further solve the crystal structure of GuApiGT, determine a key sugar-binding motif (RLGSDH) through structural analysis and theoretical calculations, and obtain mutants with altered sugar selectivity through protein engineering. Moreover, we discover 121 candidate apiosyltransferase genes from Leguminosae plants, and identify the functions of 4 enzymes. Finally, we introduce GuApiGT and its upstream genes into Nicotiana benthamiana, and complete de novo biosynthesis of a series of flavonoid apiosides. This work reports an efficient phenolic apiosyltransferase, and reveals mechanisms for its sugar donor selectivity.
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