ArticleOptics express2023
Aberration-free holographic microscope for simultaneous imaging and stimulation of neuronal populations.
Article in Optics express, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 11 citations in OpenAlex.
- Multitarget neurostimulation of the deep brain: clinical opportunities, challenges, and emerging technologies.Journal of neural engineering · 2025Review
- Article
- Testing the role of spontaneous activity in visuospatial perception with patterned optogenetics.PloS one · 2025Article
- Multicolor two-photon light-patterning microscope exploiting the spatio-temporal properties of a fiber bundle.Biomedical optics express · 2024Article
- Aberration-free holographic microscope for simultaneous imaging and stimulation of neuronal populations.Optics express · 2023Article
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Authors and funding
2 authors.
Funding
Abstract
A technical challenge in neuroscience is to record and specifically manipulate the activity of neurons in living animals. This can be achieved in some preparations with two-photon calcium imaging and photostimulation. These methods can be extended to three dimensions by holographic light sculpting with spatial light modulators (SLMs). At the same time, performing simultaneous holographic imaging and photostimulation is still cumbersome, requiring two light paths with separate SLMs. Here we present an integrated optical design using a single SLM for simultaneous imaging and photostimulation. Furthermore, we applied axially dependent adaptive optics to make the system aberration-free, and developed software for calibrations and closed-loop neuroscience experiments. Finally, we demonstrate the performance of the system with simultaneous calcium imaging and optogenetics in mouse primary auditory cortex in vivo. Our integrated holographic system could facilitate the systematic investigation of neural circuit function in awake behaving animals.
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Registered trials
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