Evidence map›Paper›PMID 37858605›Full record

ArticleCancer science2023

EpCAM and APN expression in combination with γ-H2AX as biomarkers for detecting hepatocarcinogens in rats.

Jun-Ichi Akagi, Young-Man Cho, Takeshi Toyoda, Yasuko Mizuta, Kumiko Ogawa

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cancer science, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Jun-Ichi AkagiDivision of Pathology, National Institute of Health Sciences, Kawasaki, Japan.ORCID https://orcid.org/0000-0002-9643-2662
Young-Man ChoDivision of Pathology, National Institute of Health Sciences, Kawasaki, Japan.
Takeshi ToyodaDivision of Pathology, National Institute of Health Sciences, Kawasaki, Japan.ORCID https://orcid.org/0000-0003-1646-4603
Yasuko MizutaDivision of Pathology, National Institute of Health Sciences, Kawasaki, Japan.
Kumiko OgawaDivision of Pathology, National Institute of Health Sciences, Kawasaki, Japan.ORCID https://orcid.org/0000-0002-4454-3838
National Institute of Health Sciences · JP

Funding

Japan Agency for Medical Research and Development 20ak0101092j0103
6 · The paper itself

Abstract

The phosphorylated form of histone H2AX (γ-H2AX) serves as a commonly utilized biomarker for DNA damage. Based on our previous findings, which demonstrated the formation of γ-H2AX foci as a reliable biomarker for detecting bladder carcinogens in repeated dose 28-day study in rats, we hypothesized that γ-H2AX could also function as a biomarker for detecting hepatocarcinogens. However, we found that γ-H2AX foci formation was not effectively induced by hepatocarcinogens that did not stimulate hepatocyte proliferation. Therefore, we explored alternative biomarkers to detect chemical hepatocarcinogenicity and discovered increased expressions of epithelial cell adhesion molecule (EpCAM/CD326)- and aminopeptidase N (APN/CD13) in the hepatocytes of rats administered various hepatocarcinogens. Significant increases in EpCAM- and APN-positive hepatocytes were observed for eight and five of the 10 hepatocarcinogens, respectively. Notably, five and two of them, respectively, were negative for γ-H2AX foci. These results highlight the potential of EpCAM and APN as useful biomarkers in combination with γ-H2AX for the detection of chemical hepatocarcinogenicity.

Indexed as

BiomarkersCarcinogensCD13 AntigensEpithelial Cell Adhesion MoleculePhosphoproteinsAnimalsGene Expression Regulation, NeoplasticHistonesMaleRatsBiomarkersCarcinogensCD13 AntigensEpithelial Cell Adhesion Moleculegamma-H2AX protein, ratHistonesPhosphoproteinsbiomarkercarcinogenesischemical exposurehepatocarcinogentoxicology

Identifiers

PMID37858605
PMCPMC10728002
OpenAlexW4387798315

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.