Evidence map›Paper›PMID 37856867›Full record

ArticleACS synthetic biology2023

Tuning Plant Promoters Using a Simple Split Luciferase Method to Assess Transcription Factor-DNA Interactions.

Y-M Cai, S Witham, N J Patron

Open access · hybridAbstract read
In one paragraph

Article in ACS synthetic biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.6field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Y-M CaiEngineering Biology, Earlham Institute, Norwich Research Park, Norwich NR4 7UZ, U.K.
S WithamEngineering Biology, Earlham Institute, Norwich Research Park, Norwich NR4 7UZ, U.K.ORCID 0000-0001-5071-5870
N J PatronEngineering Biology, Earlham Institute, Norwich Research Park, Norwich NR4 7UZ, U.K.ORCID 0000-0002-8389-1851
Norwich Research Park · GB

Funding

Biotechnology and Biological Sciences Research Council BB/CSP1720/1Biotechnology and Biological Sciences Research Council BB/L014130Biotechnology and Biological Sciences Research Council BB/L014130/1Biotechnology and Biological Sciences Research Council BB/M011216/1Biotechnology and Biological Sciences Research Council BBS/E/T/000PR9819
6 · The paper itself

Abstract

Sequence features, including the affinity of binding motifs for their cognate transcription factors, are important contributors to promoter behavior. The ability to predictably recode affinity enables the development of synthetic promoters with varying levels of response to known cellular signals. Here we describe a luminescence-based microplate assay for comparing the interactions of transcription factors with short DNA probes. We then demonstrate how these data can be used to design synthetic plant promoters of varying strengths that respond to the same transcription factor.

Indexed as

Gene Expression RegulationTranscription FactorsDNALuciferasesPromoter Regions, GeneticDNALuciferasesTranscription FactorsArabidopsisplant biotechnologyprotein bindingsynthetic promoterstranscription factors

Identifiers

PMID37856867
PMCPMC10661027
OpenAlexW4387769502

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.