Mucociliary Transport Device Construction and Application to Study Mucociliary Clearance.

Patrick R Sears, Lawrence E Ostrowski

Open access · greenAbstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it

0cells of the map it votes in

2citing papers in PubMed

2.4field-weighted citation impact, top 10% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 1 citations in OpenAlex.

Preclinical human models of primary ciliary dyskinesia.European respiratory review : an official journal of the European Respiratory Society · 2026
Review
Development of an inhalable contrast agent targeting the respiratory tract mucus layer for pulmonary ultrasonic imaging.Scientific reports · 2024
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Patrick R SearsMarsico Lung Institute, University of North Carolina, Chapel Hill, NC, USA.

Lawrence E OstrowskiMarsico Lung Institute, University of North Carolina, Chapel Hill, NC, USA. ostro@med.unc.edu.

Lung Institute · US

Funding

Functional Studies of Novel Genes Mutated in Primary Ciliary Dyskinesia II: Genotype to PhenotypeR01HL117836 · NHLBI · UNIV OF NORTH CAROLINA CHAPEL HILL · PI OSTROWSKI, LAWRENCE E · 2013 to 2023

$4.4M

NHLBI NIH HHS R01 HL117836

6 · The paper itself

Abstract

Well-differentiated air-liquid interface cultures of airway epithelial cells produce and secrete mucus and have abundant cilia that beat in the apical fluid. In cultures, this ciliary beating is not well coordinated or occurs in small focal areas so the resulting mucociliary transport (MCT) is only linear over short distances. We present a method which induces ciliated cells in cultures to align during growth. The cells align along the axis of a defined circular track, thus producing a well-coordinated rotational transport which is effectively linear on length scales of ciliated cells. These modified inserts - referred to as mucociliary transport devices (MCTDs) - are simple to prepare and result reproducibly in a high percentage of cultures demonstrating complete circular transport (CCT).

Indexed as

Mucociliary ClearanceRespiratory SystemCells, CulturedCiliaEpithelial CellsMucusAir-liquid interface cultureCiliary coordinationCollagen patterningFluorescence video microscopyFluorescent particle trackingMucociliary transport

Identifiers

PMID37856031

DOI10.1007/978-1-0716-3507-0_17

PMCPMC12679460

OpenAlexW4387764763

What OpenQuestion holds

Textmetadata

LicenceTDM

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.