Evidence map›Paper›PMID 37856023›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Endogenous Tagging of Ciliary Genes in Human RPE1 Cells for Live-Cell Imaging.

Stefanie Kuhns, Alice Dupont Juhl, Zeinab Anvarian, Daniel Wüstner, Lotte B Pedersen, Jens S Andersen

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
9.7field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Stefanie KuhnsDepartment of Biochemistry and Molecular Biology, University of Southern Denmark, Odense M, Denmark.
Alice Dupont JuhlDepartment of Biochemistry and Molecular Biology, University of Southern Denmark, Odense M, Denmark.
Zeinab AnvarianDepartment of Biology, University of Copenhagen, Copenhagen Ø, Denmark.
Daniel WüstnerDepartment of Biochemistry and Molecular Biology, University of Southern Denmark, Odense M, Denmark.
Lotte B PedersenDepartment of Biology, University of Copenhagen, Copenhagen Ø, Denmark.
Jens S AndersenDepartment of Biochemistry and Molecular Biology, University of Southern Denmark, Odense M, Denmark. Jens.andersen@bmb.sdu.dk.
University of Southern Denmark · DKUniversity of Copenhagen · DK

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

CRISPR-mediated endogenous tagging of genes provides unique possibilities to explore the function and dynamic subcellular localization of proteins in living cells. Here, we describe experimental strategies for endogenous PCR-tagging of ciliary genes in human RPE1 cells and how image acquisition and analysis of the expressed fluorescently tagged proteins can be utilized to study the dynamic ciliary processes of intraflagellar transport and vesicular trafficking.

Indexed as

CiliaProteinsBiological TransportHumansProtein TransportProteinsCas12aCas9CiliaCRISPRFluorescently tagged proteinsImage analysisIntraflagellar transportLive-cell imagingVesicle trafficking

Identifiers

PMID37856023
OpenAlexW4387764781

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.