Microcontact printing of choline oxidase using a polycation-functionalized zwitterionic polymer as enzyme immobilization matrix.

Ming Zhao, Yan Cao, I-Wen Huang, Harold G Monbouquette

Abstract read

In one paragraph

Article in The Analyst, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Ming ZhaoChemical and Biomolecular Engineering Department, University of California, Los Angeles, Los Angeles, CA 90095, USA. hmonbouq@ucla.edu.

Yan CaoChemical and Biomolecular Engineering Department, University of California, Los Angeles, Los Angeles, CA 90095, USA. hmonbouq@ucla.edu.

I-Wen HuangChemical and Biomolecular Engineering Department, University of California, Los Angeles, Los Angeles, CA 90095, USA. hmonbouq@ucla.edu.

Harold G MonbouquetteChemical and Biomolecular Engineering Department, University of California, Los Angeles, Los Angeles, CA 90095, USA. hmonbouq@ucla.edu.ORCID http://orcid.org/0000-0001-8144-8189

Funding

Multifunctional Microprobe for Multiple Neurotransmitter Sensing and OptogeneticsR01NS087494 · NINDS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI MAIDMENT, NIGEL T, MONBOUQUETTE, HAROLD GEORGE · 2014 to 2018

$2.5M

NINDS NIH HHS R01 NS087494

6 · The paper itself

Abstract

Highly sensitive and selective choline microbiosensors were constructed by microcontact printing (μCP) of choline oxidase (ChOx) in a crosslinked, polyamine-functionalized zwitterionic polymer matrix on microelectrode arrays (MEAs). μCP has emerged as a potential means to create implantable, multiplexed sensor microprobes, which requires the targeted deposition of different sensor materials to specific microelectrode sites on a MEA. However, the less than sufficient enzyme loading and inadequate spatial resolution achieved with current μCP approaches has limited adoption of the method for electroenzymatic microsensors. A novel polymer, poly(2-methacryloyloxyethyl phosphorylcholine)-

Indexed as

Alcohol OxidoreductasesPolymersAnimalsCholineMammalsPolyelectrolytesAlcohol OxidoreductasesCholinecholine oxidasePolyelectrolytesPolymers

Identifiers

PMID37855743

DOI10.1039/d3an01263h

PMCPMC10842005

What OpenQuestion holds

Textmetadata

LicenceTDM

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.