Evidence map›Paper›PMID 37850509›Full record

ArticleMolecular oral microbiology2024

Metabolism of serine/glycine lipids by human gingival cells in culture.

Tyler M Guido, Samuel D Ratcliffe, Amanda Rahmlow, Matthew A Zambrello, Anthony A Provates, Robert B Clark, Michael B Smith, Frank C Nichols

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular oral microbiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. The multifaceted role of c-di-AMP signaling in the regulation ofFrontiers in cellular and infection microbiology · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Tyler M GuidoDivision of Periodontology, University of Connecticut School of Dental Medicine, Farmington, Connecticut, USA.
Samuel D RatcliffeDivision of Periodontology, University of Connecticut School of Dental Medicine, Farmington, Connecticut, USA.
Amanda RahmlowDivision of Periodontology, University of Connecticut School of Dental Medicine, Farmington, Connecticut, USA.
Matthew A ZambrelloDivision of Periodontology, University of Connecticut School of Dental Medicine, Farmington, Connecticut, USA.
Anthony A ProvatesCenter for Environmental Science and Engineering, Storrs, Connecticut, USA.
Robert B ClarkDepartment of Immunology, University of Connecticut School of Medicine, Farmington, Connecticut, USA.
Michael B SmithDepartment of Chemistry, University of Connecticut, Storrs, Connecticut, USA.
Frank C NicholsDivision of Periodontology, University of Connecticut School of Dental Medicine, Farmington, Connecticut, USA.ORCID 0000-0001-6800-1112

Funding

Porphyromonas gingivalis glycine lipids mediate bone loss through TLR2R01DE027642 · NIDCR · UNIVERSITY OF CONNECTICUT SCH OF MED/DNT · PI NICHOLS, FRANK C · 2019 to 2022
$1.6M
NIDCR NIH HHS R01 DE027642
6 · The paper itself

Abstract

Porphyromonas gingivalis produces five classes of serine/glycine lipids that are recovered in lipid extracts from periodontitis-afflicted teeth and diseased gingival tissues, particularly at sites of periodontitis. Because these lipids are recovered in diseased gingival tissues, the purpose of the present study was to evaluate the capacity of cultured human gingival fibroblasts (HGF), keratinocytes, and macrophages to hydrolyze these lipids. We hypothesize that one or more of these cell types will hydrolyze the serine/glycine lipids. The primary aim was to treat these cell types for increasing time in culture with individual highly enriched serine/glycine lipid preparations. At specified times, cells and culture media samples were harvested and extracted for hydrolysis products. The serine/glycine lipids and hydrolysis products were quantified using liquid chromatography-mass spectrometry (LC-MS) and free fatty acids were quantified using gas chromatograph-mass spectrometer. LC-MS analysis used two different mass spectrometric methods. This study revealed that treatment of HGF or macrophage (THP1) cells with lipid (L) 654 resulted in breakdown to L342 and subsequent release into culture medium. However, L654 was converted only to L567 in gingival keratinocytes. By contrast, L1256 was converted to L654 by fibroblasts and macrophages but no further hydrolysis or release into medium was observed. Gingival keratinocytes showed no hydrolysis of L1256 to smaller lipid products but because L1256 was not recovered in these cells, it is not clear what hydrolysis products are produced from L1256. Although primary cultures of gingival fibroblasts and macrophages are capable of hydrolyzing specific serine/glycine lipids, prior analysis of lipid extracts from diseased gingival tissues revealed significantly elevated levels of L1256 in diseased tissues. These results suggest that the hydrolysis of bacterial lipids in gingival tissues may reduce the levels of specific lipids, but the hydrolysis of L1256 is not sufficiently rapid to prevent significant accumulation at periodontal disease sites.

Indexed as

FibroblastsGingivaGlycineKeratinocytesMacrophagesSerineCells, CulturedChromatography, LiquidCulture MediaGas Chromatography-Mass SpectrometryHumansHydrolysisLipid MetabolismLipidsMass SpectrometryPeriodontitisCulture MediaGlycineLipidsSerinefibroblastskeratinocyteslipidsmacrophagesmetabolismPorphyromonas gingivalis

Identifiers

PMID37850509
PMCPMC11024056

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.