ReviewBME frontiers2021
Recent Advancements in Optical Harmonic Generation Microscopy: Applications and Perspectives.
Review in BME frontiers, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 85 citations in OpenAlex.
- Artificial Second Harmonic Generation Image Feature Tuning with Generative Models and SeFa Semantic Analysis.JPhys photonics · 2026Article
- The extracellular matrix as a dynamic regulator of brain function and plasticity.Neurobiology of disease · 2026Review
- Chiral nonlinear polaritonics with van der Waals metasurfaces.Science advances · 2026Article
- Near-Infrared Cocrystal Nanofluorophore with Enhanced Two-Photon Absorption Cross Sections.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Scattering-enabled epi-quantitative phase imaging reveals subcellular detail in organoids and deep mouse brains.bioRxiv : the preprint server for biology · 2026Article
- Label-free mid-infrared dichroism-sensitive photoacoustic microscopy for histostructural analysis of engineered heart tissues.Light, science & applications · 2026Article
- Optical characterization of ocular collagen microstructure and anisotropy in animal and human eyes.Biomedical optics express · 2026Article
- Review of shortwave infrared imaging and spectroscopy in tissue [Invited].Biomedical optics express · 2025Review
- Label-free nonlinear microscopy probes cellular metabolism and myelin dynamics in live tissue.Communications biology · 2025Article
- Atomic Force Microscopy-Based Nanomechanical Signatures for Staging Classification and Drug Response in Pulmonary Fibrosis.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Three-dimensional quantitative micro-elastography reveals alterations in spatial elasticity patterns in murine ovaries during ageing.Communications biology · 2025Article
- Second harmonic generation for brain imaging: pathology-related studies.Biophysical reviews · 2025Article
- Integrating nonlinear optical and Raman spectral imaging for label-free pathological examinations.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2025Article
- From Molecule to Aggregate: Designing AIE Nanocrystals for Low-Power Backward Third-Harmonic Generation Angiography.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- Cervical Collagen Network Porosity Assessed by SHG Endomicroscopy Distinguishes Preterm and Normal Pregnancy-A Pilot Study.IEEE transactions on bio-medical engineering · 2025Article
- Use of optical techniques to evaluate the ionizing radiation effects on biological specimens.Journal of radiation research · 2024Review
- Polar Metallicity Controlled by Epitaxial Strain Engineering.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- SHG Fiberscopy Assessment of Collagen Morphology and Its Potential for Breast Cancer Optical Histology.IEEE transactions on bio-medical engineering · 2024Article
- Quantitative Optical Imaging of Oxygen in Brain Vasculature.The journal of physical chemistry. B · 2024Review
- [Tissue biomechanics: connective tissue characterization : Cluster tissue biomechanics].Orthopadie (Heidelberg, Germany) · 2024Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Second harmonic generation (SHG) and third harmonic generation (THG) microscopies have emerged as powerful imaging modalities to examine structural properties of a wide range of biological tissues. Although SHG and THG arise from very different contrast mechanisms, the two are complimentary and can often be collected simultaneously using a modified multiphoton microscope. In this review, we discuss the needed instrumentation for these modalities as well as the underlying theoretical principles of SHG and THG in tissue and describe how these can be leveraged to extract unique structural information. We provide an overview of recent advances showing how SHG microscopy has been used to evaluate collagen alterations in the extracellular matrix and how this has been used to advance our knowledge of cancers, fibroses, and the cornea, as well as in tissue engineering applications. Specific examples using polarization-resolved approaches and machine learning algorithms are highlighted. Similarly, we review how THG has enabled developmental biology and skin cancer studies due to its sensitivity to changes in refractive index, which are ubiquitous in all cell and tissue assemblies. Lastly, we offer perspectives and outlooks on future directions of SHG and THG microscopies and present unresolved questions, especially in terms of overall miniaturization and the development of microendoscopy instrumentation.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.