ArticleMicrobiome2023
Novel methodologies for host-microbe interactions and microbiome-targeted therapeutics in 3D organotypic skin models.
Article in Microbiome, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Recent advances in immunocompetent human skin-on-chip models: Construction strategies and biomedical applications.Bioactive materials · 2026Review
- Current research landscape and future prospects of in silico modeling approaches for atopic dermatitis.JID innovations : skin science from molecules to population health · 2026Review
- Universal-BacMicrobiologyOpen · 2026Article
- Self-Assembled Skin Equivalents with Monoclonal CRISPR/Cas9-Modified N/TERT-1 Keratinocytes: A Cutting-Edge Model for Human Skin and its Diseases.Advanced healthcare materials · 2026Article
- Development of an ex vivo skin model for quantitative recovery and preservation of donor-specific human microbiota.BMC microbiology · 2026Article
- Advancing human skin models by integrating skin microbes for next-generation research.Scientific reports · 2026Article
- Validated Step-by-Step Protocols for Human Keratinocyte Isolation and the Construction of Organotypic 3D Epidermal Models.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Harnessing Next-Generation 3D Cancer Models to Elucidate Tumor-Microbiome Crosstalk.Advanced healthcare materials · 2026Review
- Toward the Next Generation of In Silico Modeling of Dynamic Host-Microbiota Interactions in the Skin.JID innovations : skin science from molecules to population health · 2025Review
- Engineered Tissue Models to Decode Host-Microbiota Interactions.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Inflammatory Transformation of Skin Basal Cells as a Key Driver of Cutaneous Aging.International journal of molecular sciences · 2025Article
- Organoids/organs-on-chips towards biomimetic human artificial skin.Burns & trauma · 2025Review
- 3D Models Currently Proposed to Investigate Human Skin Aging and Explore Preventive and Reparative Approaches: A Descriptive Review.Biomolecules · 2024Review
- A New Method for Obtaining Monospecies and Binary Cultures of Staphylococcus spp. in Alginate Gel and the Study of the Action of Active Compounds on These Cultures on the Example of Catecholamines.Bulletin of experimental biology and medicine · 2024Article
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Corrections and comments
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Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundFollowing descriptive studies on skin microbiota in health and disease, mechanistic studies on the interplay between skin and microbes are on the rise, for which experimental models are in great demand. Here, we present a novel methodology for microbial colonization of organotypic skin and analysis thereof.
resultsAn inoculation device ensured a standardized application area on the stratum corneum and a homogenous distribution of bacteria, while preventing infection of the basolateral culture medium even during prolonged culture periods for up to 2 weeks at a specific culture temperature and humidity. Hereby, host-microbe interactions and antibiotic interventions could be studied, revealing diverse host responses to various skin-related bacteria and pathogens.
conclusionsOur methodology is easily transferable to a wide variety of organotypic skin or mucosal models and different microbes at every cell culture facility at low costs. We envision that this study will kick-start skin microbiome studies using human organotypic skin cultures, providing a powerful alternative to experimental animal models in pre-clinical research. Video Abstract.
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Registered trials
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