Evidence map›Paper›PMID 37848645›Full record

ArticleReproductive sciences (Thousand Oaks, Calif.)2024

Implication of Novel BMP15 and GDF9 Variants in Unexpected Poor Ovarian Response.

Anahita Mehdizadeh, Mansoureh Soleimani, Fatemehsadat Amjadi, Azadeh Akbari Sene, Mohammad Hassan Sheikhha, Ali Dehghani, Sareh Ashourzadeh, Bibi Shahnaz Aali, Shahriar Dabiri, Zahra Zandieh

Abstract read
PubMed Publisher
In one paragraph

Article in Reproductive sciences (Thousand Oaks, Calif.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.2field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Regulation of Curcumin on Follicle Initiation Rate in Diminished Ovarian Reserve.Combinatorial chemistry & high throughput screening · 2025
    Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 5 institutions in 2 countries.

Anahita MehdizadehDepartment of Anatomical Sciences, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Mansoureh SoleimaniDepartment of Anatomical Sciences, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Fatemehsadat AmjadiDepartment of Anatomical Sciences, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Azadeh Akbari SeneShahid Akbarabadi Clinical Research Development Unit (ShACRDU), Iran University of Medical Sciences, Tehran, Iran.
Mohammad Hassan SheikhhaDepartment of Medical Genetics, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Ali DehghaniDepartment of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Sareh AshourzadehAfzalipour Clinical Center for Infertility, Kerman University of Medical Sciences, Kerman, Iran.
Bibi Shahnaz AaliFRANZCOG Rockingham Peel Group, South Metropolitan Health Service, Murdoch, Australia.
Shahriar DabiriFaculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
Zahra ZandiehDepartment of Anatomical Sciences, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. zandie.dvm@gmail.com.ORCID 0000-0002-7720-9465
Iran University of Medical Sciences · IRKerman University of Medical Sciences · IRNorth Metropolitan Health Service · AUShahid Sadoughi University of Medical Sciences and Health Services · IRTarbiat Modares University · IR

Funding

Iran University of Medical Sciences 99-3-4-19128
6 · The paper itself

Abstract

Unexpected poor ovarian response (UPOR) occurs when nine or fewer oocytes are retrieved from a young patient with normal ovarian reserve. Bone morphogenetic protein15 (BMP15) and growth differentiation factor 9 (GDF9) are two oocyte-specific factors with pivotal role in folliculogenesis. The aim of this study was to assess the relation between BMP15 and GDF9 variants with UPOR. Hundred women aged ≤ 39 with AMH ≥ 1.27 IU/ml participated as UPOR and normal ovarian responders (NOR) based on their oocyte number. Each group consisted of 50 patients. After genomic DNA extraction, the entire exonic regions of BMP15 and GDF9 were amplified and examined by direct sequencing. Western blotting was performed to determine the expression levels of BMP15 and GDF9 in follicular fluid. Additionally, in silico analysis was applied to predict the effect of discovered mutations. From four novel variants of BMP15 and GDF9 genes, silent mutations (c.744 T > C) and (c.99G > A) occurred in both groups, whereas missense variants: c.967-968insA and c.296A > G were found exclusively in UPORs. The latter variants caused reduction in protein expression. Moreover, the mutant allele (T) in a GDF9 polymorphism (C447T) found to be more in NOR individuals (58% NOR vs. 37% UPOR (OR = 2.3, CI 1.32-4.11, p = 0.004).The novel missense mutations which were predicted as damaging, along with other mutations that happened in UPORs might result in ovarian resistance to stimulation. The mutant allele (T) in C447T polymorphism has a protective effect. It can be concluded that there is an association between BMP15 and GDF9 variants and follicular development and ovarian response.

Indexed as

Bone Morphogenetic Protein 15Growth Differentiation Factor 9FemaleFollicular FluidHumansOocytesOvaryBMP15 protein, humanBone Morphogenetic Protein 15GDF9 protein, humanGrowth Differentiation Factor 9BMP15GDF9POSEIDON criteriaSequencingUnexpected poor ovarian response

Identifiers

PMID37848645
OpenAlexW4387697506

What OpenQuestion holds

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Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.