Evidence map›Paper›PMID 37847071›Full record

ArticleDevelopmental dynamics : an official publication of the American Association of Anatomists2024

Loss of function of male-specific lethal 3 (Msl3) does not affect spermatogenesis in rodents.

T A Mitchell, J M Lin, S M Hicks, J R James, P Rangan, P E Forni

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Developmental dynamics : an official publication of the American Association of Anatomists, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.6field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

T A MitchellDepartment of Biological Sciences, University at Albany, State University of New York, Albany, New York, USA.ORCID 0000-0002-9647-6024
J M LinDepartment of Biological Sciences, University at Albany, State University of New York, Albany, New York, USA.ORCID 0000-0002-9197-0816
S M HicksDepartment of Biological Sciences, University at Albany, State University of New York, Albany, New York, USA.ORCID 0000-0003-4041-8105
J R JamesDepartment of Biological Sciences, University at Albany, State University of New York, Albany, New York, USA.
P RanganBlack Family Stem Cell Institute, Department of Cell, Developmental, and Regenerative Biology, Icahn School of Medicine at Mount Sinai, New York, New York, USA.ORCID 0000-0002-1452-8119
P E ForniDepartment of Biological Sciences, University at Albany, State University of New York, Albany, New York, USA.ORCID 0000-0001-6547-3464
Albany State University · USIcahn School of Medicine at Mount Sinai · US

Funding

Regulation of stem cell differentiation during Drosophila oogenesisR01GM111779 · NIGMS · STATE UNIVERSITY OF NEW YORK AT ALBANY · PI RANGAN, PRASHANTH · 2014 to 2022
$2.7M
Understanding the role of the transcription factor Gli3 in Kallmann syndrome and normosmic forms of idiopathic hypogonadotropic hypogonadism.R01HD097331 · NICHD · STATE UNIVERSITY OF NEW YORK AT ALBANY · PI Paolo E Forni · 2019 to 2026
$2.2M
Molecular Mechanisms Controlling Differentiation and Circuit Formation of Vomeronasal Sensory NeuronsR01DC017149 · NIDCD · STATE UNIVERSITY OF NEW YORK AT ALBANY · PI FORNI, PAOLO E · 2019 to 2023
$1.8M
Role of RNA degradation in germline stem cell to oocyte transitionR01GM135628 · NIGMS · STATE UNIVERSITY OF NEW YORK AT ALBANY · PI RANGAN, PRASHANTH · 2020 to 2023
$1.3M
NICHD NIH HHS R01 HD097331NIDCD NIH HHS R01 DC017149NIGMS NIH HHS R01 GM111779NIGMS NIH HHS R01 GM135628NIGMS NIH HHS RO1GM11177NIGMS NIH HHS RO1GM135628
6 · The paper itself

Abstract

backgroundMale-specific lethal 3 (Msl3) is a member of the chromatin-associated male-specific lethal MSL complex, which is responsible for the transcriptional upregulation of genes on the X chromosome in males of Drosophila. Although the dosage complex operates differently in mammals, the Msl3 gene is conserved from flies to humans. Msl3 is required for meiotic entry during Drosophila oogenesis. Recent reports indicate that also in primates, Msl3 is expressed in undifferentiated germline cells before meiotic entry. However, if Msl3 plays a role in the meiotic entry of mammals has yet to be explored.

resultsTo understand, if Msl3a plays a role in the meiotic entry of mammals, we used mouse spermatogenesis as a study model. Analyses of single-cell RNA-seq data revealed that, in mice, Msl3 is mostly expressed in meiotic cells. To test the role of Msl3 in meiosis, we used a male germline-specific Stra8-iCre driver and a newly generated Msl3

conclusionsOur data suggest that, in mice, Msl3 exhibits delayed expression compared to Drosophila and primates, and loss-of-function mutations disrupting the chromodomain of Msl3 alone do not impede meiotic entry in rodents.

Indexed as

MeiosisSpermatogenesisAnimalsChromosomal Proteins, Non-HistoneDNA-Binding ProteinsFemaleLoss of Function MutationMaleMiceMice, KnockoutRNA-Binding ProteinsChromosomal Proteins, Non-HistoneDNA-Binding ProteinsMsl3 protein, mouseRNA-Binding Proteinsmale‐specific lethal 3 (Msl3)meiosismeiotic entryspermatogenesis

Identifiers

PMID37847071
PMCPMC11021377
OpenAlexW4387692007

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.