Evidence map›Paper›PMID 37843624›Full record

ArticleInternational journal of legal medicine2024

Internal validation of the GA118-24B Genetic Analyzer, a stable capillary electrophoresis system for forensic DNA identification.

Weifeng Qu, Feng Wang, Dan Sun, Yi Liu, Xin Jin, Zheng Gong, Jinjie Liu, Ningjie Zhang, Dan Wen, Chudong Wang and 15 more

Abstract read
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In one paragraph

Article in International journal of legal medicine, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

25 authors.

Weifeng QuDepartment of Forensic Science, School of Basic Medical Sciences, Central South University, No. 172. Tongzipo Road, Changsha, 410013, China.
Feng WangMinistry of Public Security of P.R.C., The First Research Institute, No. 1. Shouti South Road, Beijing, 100044, China.
Dan SunMinistry of Public Security of P.R.C., The First Research Institute, No. 1. Shouti South Road, Beijing, 100044, China.
Yi LiuDepartment of Forensic Science, School of Basic Medical Sciences, Central South University, No. 172. Tongzipo Road, Changsha, 410013, China.
Xin JinPublic Security Department of Hainan Province, No. 9. Binya Road, Hainan, 570203, China.
Zheng GongCriminal Technology Detachment, Public Security Bureau of Harbin, No. 35. Dongfeng Road, Harbin, 150010, China.
Jinjie LiuCriminal Investigation Detachment, Public Security Bureau of Beijing, No. 44. Banbuqiao Road, Beijing, 102611, China.
Ningjie ZhangMinistry of Public Security of P.R.C., The First Research Institute, No. 1. Shouti South Road, Beijing, 100044, China.
Dan WenDepartment of Forensic Science, School of Basic Medical Sciences, Central South University, No. 172. Tongzipo Road, Changsha, 410013, China.
Chudong WangDepartment of Forensic Science, School of Basic Medical Sciences, Central South University, No. 172. Tongzipo Road, Changsha, 410013, China.
Hongtao JiaDepartment of Forensic Science, School of Basic Medical Sciences, Central South University, No. 172. Tongzipo Road, Changsha, 410013, China.
Ruyi XuDepartment of Forensic Science, School of Basic Medical Sciences, Central South University, No. 172. Tongzipo Road, Changsha, 410013, China.
Xuan TangDepartment of Forensic Science, School of Basic Medical Sciences, Central South University, No. 172. Tongzipo Road, Changsha, 410013, China.
Siqi ChenDepartment of Forensic Science, School of Basic Medical Sciences, Central South University, No. 172. Tongzipo Road, Changsha, 410013, China.
Xiaoyi FuDepartment of Forensic Science, School of Basic Medical Sciences, Central South University, No. 172. Tongzipo Road, Changsha, 410013, China.
Xue LiDepartment of Forensic Science, School of Basic Medical Sciences, Central South University, No. 172. Tongzipo Road, Changsha, 410013, China.
Haibo RongMinistry of Public Security of P.R.C., The First Research Institute, No. 1. Shouti South Road, Beijing, 100044, China.
Tao ZhangMinistry of Public Security of P.R.C., The First Research Institute, No. 1. Shouti South Road, Beijing, 100044, China.
Chuan JinMinistry of Public Security of P.R.C., The First Research Institute, No. 1. Shouti South Road, Beijing, 100044, China.
Li ChenMinistry of Public Security of P.R.C., The First Research Institute, No. 1. Shouti South Road, Beijing, 100044, China.
Jienan LiDepartment of Forensic Science, School of Basic Medical Sciences, Central South University, No. 172. Tongzipo Road, Changsha, 410013, China.
Ying LiuDepartment of Forensic Science, School of Basic Medical Sciences, Central South University, No. 172. Tongzipo Road, Changsha, 410013, China.
Jifeng CaiDepartment of Forensic Science, School of Basic Medical Sciences, Central South University, No. 172. Tongzipo Road, Changsha, 410013, China.
Bowei Jiang *Ministry of Public Security of P.R.C., The First Research Institute, No. 1. Shouti South Road, Beijing, 100044, China. jiangbw@fiscan.cn.
Lagabaiyila Zha *Department of Forensic Science, School of Basic Medical Sciences, Central South University, No. 172. Tongzipo Road, Changsha, 410013, China. dlkl2001@163.com.ORCID http://orcid.org/0000-0002-7900-122X

Funding

joint postgraduate training base between Central South University and the First Research Institute of the Ministry of Public Security of P.R.C 2020pyjd23National Key Research and Development Program 2021YFC3300104National Natural Science Foundation of China 82230064National Natural Science Foundation of China 82271928
6 · The paper itself

Abstract

The GA118-24B Genetic Analyzer (hereafter, "GA118-24B") is an independently developed capillary electrophoresis instrument. In the present research, we designed a series of validation experiments to test its performance at detecting DNA fragments compared to the Applied Biosystems 3500 Genetic Analyzer (hereafter, "3500"). Three commercially available autosomal short tandem repeat multiplex kits were used in this validation. The results showed that GA118-24B had acceptable spectral calibration for three kits. The results of accuracy and concordance studies were also satisfactory. GA118-24B showed excellent precision, with a standard deviation of less than 0.1 bp. Sensitivity and mixture studies indicated that GA118-24B could detect low-template DNA and complex mixtures as well as the results generated by 3500 in parallel experiments. Based on the experimental results, we set specific analytical and stochastic thresholds. Besides, GA118-24B showed superiority than 3500 within certain size ranges in the resolution study. Instead of conventional commercial multiplex kits, GA118-24B performed stably on a self-developed eight-dye multiplex system, which were not performed on 3500 Genetic Analyzer. We compared our validation results with those of previous research and found our results to be convincing. Overall, we conclude that GA118-24B is a stable and reliable genetic analyzer for forensic DNA identification.

Indexed as

DNADNA FingerprintingElectrophoresis, CapillaryHumansMicrosatellite RepeatsPolymerase Chain ReactionDNAGA118-24B Genetic AnalyzerInternal validationPolymerase chain reactionShort tandem repeat

Identifiers

PMID37843624

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.