Evidence map›Paper›PMID 37843021›Full record

ArticleNano letters2023

Reversible, Covalent DNA Condensation Approach Using Chemical Linkers for Enhanced Gene Delivery.

Wenliang Wang, Aaron Tasset, Ilya Pyatnitskiy, Peter Lin, Arjun Bellamkonda, Rohan Mehta, Christian Gabbert, Feng Yuan, Heba Galaa Mohamed, Nicholas A Peppas and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nano letters, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Wenliang WangBiomedical Engineering Cockrell School of Engineering, The University of Texas at Austin, Austin, Texas 78712, United States.ORCID 0000-0003-0575-8139
Aaron TassetBiomedical Engineering Cockrell School of Engineering, The University of Texas at Austin, Austin, Texas 78712, United States.
Ilya PyatnitskiyBiomedical Engineering Cockrell School of Engineering, The University of Texas at Austin, Austin, Texas 78712, United States.
Peter LinBiomedical Engineering Cockrell School of Engineering, The University of Texas at Austin, Austin, Texas 78712, United States.
Arjun BellamkondaBiomedical Engineering Cockrell School of Engineering, The University of Texas at Austin, Austin, Texas 78712, United States.
Rohan MehtaBiomedical Engineering Cockrell School of Engineering, The University of Texas at Austin, Austin, Texas 78712, United States.
Christian GabbertBiomedical Engineering Cockrell School of Engineering, The University of Texas at Austin, Austin, Texas 78712, United States.
Feng YuanBiomedical Engineering Cockrell School of Engineering, The University of Texas at Austin, Austin, Texas 78712, United States.
Heba Galaa MohamedBiomedical Engineering Cockrell School of Engineering, The University of Texas at Austin, Austin, Texas 78712, United States.
Nicholas A PeppasBiomedical Engineering Cockrell School of Engineering, The University of Texas at Austin, Austin, Texas 78712, United States.ORCID 0000-0003-0894-5626
Huiliang WangBiomedical Engineering Cockrell School of Engineering, The University of Texas at Austin, Austin, Texas 78712, United States.ORCID 0000-0003-4063-270X

Funding

Non-Invasive and Non-Viral Sono-OptogeneticsR35GM147408 · NIGMS · UNIVERSITY OF TEXAS AT AUSTIN · PI Huiliang Wang · 2022 to 2026
$2.0M
NIGMS NIH HHS R35 GM147408
6 · The paper itself

Abstract

Nonviral gene delivery has emerged as a promising technology for gene therapy. Nonetheless, these approaches often face challenges, primarily associated with lower efficiency, which can be attributed to the inefficient transportation of DNA into the nucleus. Here, we report a two-stage condensation approach to achieve efficient nuclear transport of DNA. First, we utilize chemical linkers to cross-link DNA plasmids via a reversible covalent bond to form smaller-sized bundled DNA (b-DNA). Then, we package the b-DNA into cationic vectors to further condense b-DNA and enable efficient gene delivery to the nucleus. We demonstrate clear improvements in the gene transfection efficiency

Indexed as

DNA, B-FormDNAGenetic TherapyGene Transfer TechniquesPlasmidsTransfectionDNADNA, B-Formbundled DNAGene deliverynonviralnuclear importreversible

Identifiers

PMID37843021
PMCPMC10932748

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.