Evidence map›Paper›PMID 37843014›Full record

ArticleACS synthetic biology2023

Combining Fusion of Cells with CRISPR-Cas9 Editing for the Cloning of Large DNA Fragments or Complete Bacterial Genomes in Yeast.

Gabrielle Guesdon, Géraldine Gourgues, Fabien Rideau, Thomas Ipoutcha, Lucía Manso-Silván, Matthieu Jules, Pascal Sirand-Pugnet, Alain Blanchard, Carole Lartigue

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS synthetic biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.9field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Gabrielle GuesdonUniv. Bordeaux, INRAE, Biologie du Fruit et Pathologie, UMR 1332, F-33140 Villenave d'Ornon, France.
Géraldine GourguesUniv. Bordeaux, INRAE, Biologie du Fruit et Pathologie, UMR 1332, F-33140 Villenave d'Ornon, France.
Fabien RideauUniv. Bordeaux, INRAE, Biologie du Fruit et Pathologie, UMR 1332, F-33140 Villenave d'Ornon, France.
Thomas IpoutchaUniv. Bordeaux, INRAE, Biologie du Fruit et Pathologie, UMR 1332, F-33140 Villenave d'Ornon, France.
Lucía Manso-SilvánCIRAD, UMR ASTRE, F-34398 Montpellier, France.
Matthieu JulesUniversité Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, F-78350 Jouy-en-Josas, France.ORCID 0000-0003-0817-8949
Pascal Sirand-PugnetUniv. Bordeaux, INRAE, Biologie du Fruit et Pathologie, UMR 1332, F-33140 Villenave d'Ornon, France.ORCID 0000-0003-2613-0762
Alain BlanchardUniv. Bordeaux, INRAE, Biologie du Fruit et Pathologie, UMR 1332, F-33140 Villenave d'Ornon, France.
Carole LartigueUniv. Bordeaux, INRAE, Biologie du Fruit et Pathologie, UMR 1332, F-33140 Villenave d'Ornon, France.ORCID 0000-0001-5550-7579
Université de Bordeaux · FRAgroParisTech · FRCentre de Coopération Internationale en Recherche Agronomique pour le Développement · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The genetic engineering of genome fragments larger than 100 kbp is challenging and requires both specific methods and cloning hosts. The yeast

Indexed as

CRISPR-Cas SystemsSaccharomyces cerevisiaeCloning, MolecularDNAGene EditingGenome, BacterialPhylogenyDNAcell fusionCReasPy-FusionCRISPR-Cas9genome editinggenome fragment capturegenome transplantationin-yeast genome cloningmembrane nuclease MnuAMycoplasma sppSaccharomyces cerevisiaewhole genome transfer

Identifiers

PMID37843014
PMCPMC10662353
OpenAlexW4387661913

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.