ArticleISME communications2023
Establishment of a transparent soil system to study Bacillus subtilis chemical ecology.
Article in ISME communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 14 citations in OpenAlex.
- Synthetic microbial communities for studying the fate and ecological role of specialized metabolites.Essays in biochemistry · 2026Review
- Synthetic soils for ecological and synthetic biology applications.FEMS microbiology reviews · 2026Review
- Evaluation of Root Films withPlants (Basel, Switzerland) · 2025Article
- Hydrogel-based experimental models of the gastrointestinal tract.Microbiome · 2025Review
- Seeing between the lines: matrix-assisted laser desorption/ionization mass spectrometry imaging advances for microbial interactions.Current opinion in microbiology · 2025Review
- Comparing field and lab quantitative stable isotope probing for nitrogen assimilation in soil microbes.Applied and environmental microbiology · 2025Article
- Surfactin facilitates establishment of Bacillus subtilis in synthetic communities.The ISME journal · 2025Article
- Resistance towards and biotransformation of a Pseudomonas-produced secondary metabolite during community invasion.The ISME journal · 2024Article
- Microbe-cellulose hydrogels as a model system for particulate carbon degradation in soil aggregates.ISME communications · 2024Article
- Construction and characterisation of a structured, tuneable, and transparent 3D culture platform for soil bacteria.Microbiology (Reading, England) · 2024Article
- Complex extracellular biology drives surface competition during colony expansion in Bacillus subtilis.The ISME journal · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Bacterial secondary metabolites are structurally diverse molecules that drive microbial interaction by altering growth, cell differentiation, and signaling. Bacillus subtilis, a Gram-positive soil-dwelling bacterium, produces a wealth of secondary metabolites, among them, lipopeptides have been vastly studied by their antimicrobial, antitumor, and surfactant activities. However, the natural functions of secondary metabolites in the lifestyles of the producing organism remain less explored under natural conditions, i.e. in soil. Here, we describe a hydrogel-based transparent soil system to investigate B. subtilis chemical ecology under controllable soil-like conditions. The transparent soil matrix allows the growth of B. subtilis and other isolates gnotobiotically and under nutrient-controlled conditions. Additionally, we show that transparent soil allows the detection of lipopeptides production and dynamics by HPLC-MS, and MALDI-MS imaging, along with fluorescence imaging of 3-dimensional bacterial assemblages. We anticipate that this affordable and highly controllable system will promote bacterial chemical ecology research and help to elucidate microbial interactions driven by secondary metabolites.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.