Evidence map›Paper›PMID 37837279›Full record

ArticleAnimal biotechnology2023

Met stimulates ARID1A degradation and activation of the PI3K-SREBP1 signaling to promote milk fat synthesis in bovine mammary epithelial cells.

Hao Qi, Gang Lin, Siqi Guo, Xudong Guo, Congying Yu, Minghui Zhang, Xuejun Gao

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Animal biotechnology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.8field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Hao QiGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, College of Life Science and Engineering, Foshan University, Foshan, Guangdong, China.
Gang LinCollege of Animal Science, Yangtze University, Jingzhou, Hubei, China.
Siqi GuoCollege of Life Science, Northeast Agricultural University, Harbin, Heilongjiang, China.
Xudong GuoCollege of Animal Science, Yangtze University, Jingzhou, Hubei, China.
Congying YuGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, College of Life Science and Engineering, Foshan University, Foshan, Guangdong, China.
Minghui ZhangCollege of Animal Science, Yangtze University, Jingzhou, Hubei, China.ORCID 0000-0002-8253-1379
Xuejun GaoGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, College of Life Science and Engineering, Foshan University, Foshan, Guangdong, China.ORCID 0000-0002-8241-6346
Foshan University · CNYangtze University · CNNortheast Agricultural University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Methionine (Met) can promote milk fat synthesis in bovine mammary epithelial cells (BMECs), but the potential molecular mechanism is largely unknown. In this report, we aim to explore the role and molecular mechanism of AT-rich interaction domain 1A (ARID1A) in milk fat synthesis stimulated by Met. ARID1A knockdown and activation indicated that ARID1A negatively regulated the synthesis of triglycerides, cholesterol and free fatty acids and the formation of lipid droplets in BMECs. ARID1A also negatively regulated the phosphorylation of PI3K and AKT proteins, as well as the expression and maturation of SREBP1. Met stimulated the phosphorylation of PI3K and AKT proteins, as well as the expression and maturation of SREBP1, while ARID1A gene activation blocked the stimulatory effects of Met. We further found that ARID1A was located in the nucleus of BMECs, and Met reduced the nuclear localization and expression of ARID1A. ARID1A gene activation blocked the stimulation of

Indexed as

MethioninePhosphatidylinositol 3-KinasesAnimalsCattleEpithelial CellsMammary Glands, AnimalMilkProto-Oncogene Proteins c-aktRacemethionineSignal TransductionMethioninePhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktRacemethionineARID1Abovine mammary epithelial cellsMetmilk fatSREBP1

Identifiers

PMID37837279
PMCPMC13353394
OpenAlexW4387637034

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.