ArticleMolecules (Basel, Switzerland)2023
A Rapid and Quantitative Method for Determining Seed Viability Using 2,3,5-Triphenyl Tetrazolium Chloride (TTC): With the Example of Wheat Seed.
Article in Molecules (Basel, Switzerland), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 26 citations in OpenAlex.
- Intra-Relations of Biochemical Profiles Defining the Germination Potential of Medium-Term Cold-Stored Maize (Plants (Basel, Switzerland) · 2026Article
- Rapid staining protocol for electroporated potato models: a novel approach for evaluating pulsed field ablation lesions.Journal of interventional cardiac electrophysiology : an international journal of arrhythmias and pacing · 2026Article
- Boosting NADPH with GABA: Converting aged seeds to functional soybean sprouts.Food chemistry: X · 2026Article
- Synergistic mitigation of lead [Pb(II)] stress in Triticum aestivum L. by heavy metal-tolerant plant growth-promoting (HMT-PGP) microbes and N-acetylcysteine (NAC).Plant cell reports · 2026Article
- What a Difference a Water Molecule Makes-A Combined Experimental/Theoretical Study on 2,3,5-triphenyl-2Molecules (Basel, Switzerland) · 2025Article
- Bacillus amyloliquefaciens YB1701 Enhances Rice Tolerance to Saline-Alkali Stress Via ROS Scavenging and Ion Homeostasis.Current microbiology · 2025Article
- Integrated transcriptomics and metabolomics to explore the mechanisms of Elaeagnus mollis diels seed viability decline.BMC genomics · 2025Article
- Unraveling the Mechanistic Basis for Control of Seed Longevity.Plants (Basel, Switzerland) · 2025Review
- Complex Seed Dormancy inPlants (Basel, Switzerland) · 2025Article
- Exploring the Biocontrol Potential ofJournal of fungi (Basel, Switzerland) · 2024Article
- Effects of Aerated Drip Irrigation on the Soil Nitrogen Distribution, Crop Growth, and Yield of Chili Peppers.Plants (Basel, Switzerland) · 2024Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Current colorimetric methods for quantitative determination of seed viability (SV) with 2,3,5-triphenyl tetrazolium chloride (TTC) have been plagued by issues of being cumbersome and time-consuming during the experimental process, slow in extraction and staining, and exhibiting inconsistent results. In this work, we introduced a new approach that combines TTC-staining with high-temperature extraction using dimethyl sulfoxide (DMSO). The optimization of the germination stage, TTC-staining method, and 1,3,5-triphenylformazan (TTF) extraction method were meticulously carried out as follows: When the majority of wheat seeds had grown the radicle, and the length of radicles was approximately equal to the seed length (24 h-germination), 2 g germinating seeds were placed into a beaker (20 mL) containing 5 mL 10 g·L
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