Evidence map›Paper›PMID 37834144›Full record

ArticleInternational journal of molecular sciences2023

The Generation of a Nanobody-Based ELISA for Human Microsomal Epoxide Hydrolase.

Qiyi He, Mark R McCoy, Meng Qi, Christophe Morisseau, Huiyi Yang, Chengpeng Xu, Rachel Shey, Michael C Goodman, Suqing Zhao, Bruce D Hammock

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.7field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 2 countries.

Qiyi HeDepartment of Entomology and Nematology and University of California Davis Comprehensive Cancer Center, University of California Davis, Davis, CA 95616, USA.
Mark R McCoyDepartment of Entomology and Nematology and University of California Davis Comprehensive Cancer Center, University of California Davis, Davis, CA 95616, USA.
Meng QiDepartment of Entomology and Nematology and University of California Davis Comprehensive Cancer Center, University of California Davis, Davis, CA 95616, USA.
Christophe MorisseauDepartment of Entomology and Nematology and University of California Davis Comprehensive Cancer Center, University of California Davis, Davis, CA 95616, USA.ORCID 0000-0002-5672-6631
Huiyi YangDepartment of Entomology and Nematology and University of California Davis Comprehensive Cancer Center, University of California Davis, Davis, CA 95616, USA.
Chengpeng XuDepartment of Entomology and Nematology and University of California Davis Comprehensive Cancer Center, University of California Davis, Davis, CA 95616, USA.
Rachel SheyDepartment of Entomology and Nematology and University of California Davis Comprehensive Cancer Center, University of California Davis, Davis, CA 95616, USA.
Michael C GoodmanDepartment of Entomology and Nematology and University of California Davis Comprehensive Cancer Center, University of California Davis, Davis, CA 95616, USA.
Suqing ZhaoDepartment of Pharmaceutical Engineering, School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, China.
Bruce D HammockDepartment of Entomology and Nematology and University of California Davis Comprehensive Cancer Center, University of California Davis, Davis, CA 95616, USA.ORCID 0000-0003-1408-8317
University of California, Davis · USGuangdong University of Technology · CN

Funding

Workshop on Environmental Technology Transfer and EntrepreneurshipP42ES004699 · NIEHS · UNIVERSITY OF CALIFORNIA DAVIS · PI HAMMOCK, BRUCE D · 1987 to 2021
$50.1M
Supplement for bioactive lipids as effectors and indicators of the deleterious effects of human healthR35ES030443 · NIEHS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI MORISSEAU, CHRISTOPHE HP · 2019 to 2025
$5.8M
NIEHS NIH HHS P42 ES004699NIEHS NIH HHS R35 ES030443
6 · The paper itself

Abstract

A microsomal epoxide hydrolase (mEH) metabolizes in vivo in both xenobiotic and endogenous epoxides associated with signaling function. Findings in patients suggest that mEH might be a biomarker for several diseases, including metastatic cancer and viral hepatitis. To easily quantify mEH, nanobodies specific to the human mEH were isolated from a phage library of llama VHHs. Four unique clones were obtained and used for developing ELISAs. Three formats of double antibody sandwich assays were investigated using different detection strategies. Using PolyHRP, the signal was strongly amplified, yielding a 22-fold lower LOD (12 pg mL

Indexed as

Epoxide HydrolasesSingle-Domain AntibodiesAntibodiesEnzyme-Linked Immunosorbent AssayEpoxy CompoundsHumansAntibodiesEpoxide HydrolasesEpoxy CompoundsSingle-Domain AntibodiesbiomarkerELISAmicrosomal epoxide hydrolasenanobody

Identifiers

PMID37834144
PMCPMC10572367
OpenAlexW4387137817

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.