ArticleInternational journal of molecular sciences2023
Transcriptomic Profiling of Embryogenic and Non-Embryogenic Callus Provides New Insight into the Nature of Recalcitrance in Cannabis.
Article in International journal of molecular sciences, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
11 citing papers in PubMed, 25 citations in OpenAlex.
- Epigenome Editing for Cannabinoid Yield: Targets, Tools, and Priorities inInternational journal of molecular sciences · 2026Review
- Doubled Haploid Production inPlants (Basel, Switzerland) · 2025Article
- A novel protocol for protoplast isolation, transfection, and culture in Cannabis sativa L.BMC plant biology · 2025Article
- Cloning ofInternational journal of molecular sciences · 2025Article
- Establishment of Genetic Transformation System of Non-Embryogenic Callus inCurrent issues in molecular biology · 2025Article
- Overexpression of PeonyPlants (Basel, Switzerland) · 2025Article
- Mediated Transformation of Tamarillo (Plants (Basel, Switzerland) · 2025Article
- Assessment of potential candidate genes for partial resistance to Sclerotinia stem rot caused by Sclerotinia sclerotiorum using real-time quantitative PCR.The plant genome · 2025Article
- Machine learning-enhanced multi-trait genomic prediction for optimizing cannabinoid profiles in cannabis.The Plant journal : for cell and molecular biology · 2025Article
- Integration analysis of transcriptome and proteome profiles brings new insights of somatic embryogenesis of two eucalyptus species.BMC plant biology · 2024Article
- Enhancing petunia tissue culture efficiency with machine learning: A pathway to improved callogenesis.PloS one · 2023Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Differential gene expression profiles of various cannabis calli including non-embryogenic and embryogenic (i.e., rooty and embryonic callus) were examined in this study to enhance our understanding of callus development in cannabis and facilitate the development of improved strategies for plant regeneration and biotechnological applications in this economically valuable crop. A total of 6118 genes displayed significant differential expression, with 1850 genes downregulated and 1873 genes upregulated in embryogenic callus compared to non-embryogenic callus. Notably, 196 phytohormone-related genes exhibited distinctly different expression patterns in the calli types, highlighting the crucial role of plant growth regulator (PGRs) signaling in callus development. Furthermore, 42 classes of transcription factors demonstrated differential expressions among the callus types, suggesting their involvement in the regulation of callus development. The evaluation of epigenetic-related genes revealed the differential expression of 247 genes in all callus types. Notably, histone deacetylases, chromatin remodeling factors, and EMBRYONIC FLOWER 2 emerged as key epigenetic-related genes, displaying upregulation in embryogenic calli compared to non-embryogenic calli. Their upregulation correlated with the repression of embryogenesis-related genes, including LEC2, AGL15, and BBM, presumably inhibiting the transition from embryogenic callus to somatic embryogenesis. These findings underscore the significance of epigenetic regulation in determining the developmental fate of cannabis callus. Generally, our results provide comprehensive insights into gene expression dynamics and molecular mechanisms underlying the development of diverse cannabis calli. The observed repression of auxin-dependent pathway-related genes may contribute to the recalcitrant nature of cannabis, shedding light on the challenges associated with efficient cannabis tissue culture and regeneration protocols.
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Registered trials
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