Evidence map›Paper›PMID 37833714›Full record

ArticleBMC biology2023

Characterization of essential eggshell proteins from Aedes aegypti mosquitoes.

Jun Isoe, Carter J Simington, Max E Oscherwitz, Alyssa J Peterson, Alberto A Rascón, Brooke B Massani, Roger L Miesfeld, Michael A Riehle

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
4.9field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 17 citations in OpenAlex.

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  11. Activation of the G protein-coupled sulfakinin receptor inhibits blood meal intake in the mosquito Aedes aegypti.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Jun IsoeDepartment of Entomology, The University of Arizona, Tucson, AZ, 85721, USA. jisoe@ag.arizona.edu.ORCID http://orcid.org/0000-0001-5630-2129
Carter J SimingtonDepartment of Chemistry and Biochemistry, The University of Arizona, Tucson, AZ, 85721, USA.
Max E OscherwitzDepartment of Chemistry and Biochemistry, The University of Arizona, Tucson, AZ, 85721, USA.
Alyssa J PetersonDepartment of Chemistry and Biochemistry, The University of Arizona, Tucson, AZ, 85721, USA.
Alberto A RascónDepartment of Chemistry, San José State University, San José, CA, 95192, USA.
Brooke B MassaniDepartment of Chemistry and Biochemistry, The University of Arizona, Tucson, AZ, 85721, USA.
Roger L MiesfeldDepartment of Chemistry and Biochemistry, The University of Arizona, Tucson, AZ, 85721, USA.
Michael A RiehleDepartment of Entomology, The University of Arizona, Tucson, AZ, 85721, USA. mriehle@ag.arizona.edu.
University of Arizona · USSan Jose State University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundUp to 40% of the world population live in areas where mosquitoes capable of transmitting the dengue virus, including Aedes aegypti, coexist with humans. Understanding how mosquito egg development and oviposition are regulated at the molecular level may provide new insights into novel mosquito control strategies. Previously, we identified a protein named eggshell organizing factor 1 (EOF1) that when knocked down with RNA interference (RNAi) resulted in non-melanized and fragile eggs that did not contain viable embryos.

resultsIn this current study, we performed a comprehensive RNAi screen of putative A. aegypti eggshell proteins to identify additional proteins that interact with intracellular EOF1. We identified several proteins essential for eggshell formation in A. aegypti and characterized their phenotypes through a combination of molecular and biochemical approaches. We found that Nasrat, Closca, and Polehole structural proteins, together with the Nudel serine protease, are indispensable for eggshell melanization and egg viability. While all four proteins are predominantly expressed in ovaries of adult females, Nudel messenger RNA (mRNA) expression is highly upregulated in response to blood feeding. Furthermore, we identified four additional secreted eggshell enzymes that regulated mosquito eggshell formation and melanization. These enzymes included three dopachrome-converting enzymes (DCEs) and one cysteine protease. All eight of these eggshell proteins were essential for proper eggshell formation. Interestingly, their eggshell surface topologies in response to RNAi did not phenocopy the effect of RNAi-EOF1, suggesting that additional mechanisms may influence how EOF1 regulates eggshell formation and melanization.

conclusionsWhile our studies did not identify a definitive regulator of EOF1, we did identify eight additional proteins involved in mosquito eggshell formation that may be leveraged for future control strategies.

Indexed as

AedesAnimalsEgg ProteinsFemaleHumansOvaryRNA Interferencechorion proteinsEgg ProteinsEggshellMelanizationMosquitoReproductionRNA interference

Identifiers

PMID37833714
PMCPMC10576393
OpenAlexW4387613243

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.