ReviewThe Plant journal : for cell and molecular biology2024
Genomic consequences associated with Agrobacterium-mediated transformation of plants.
Review in The Plant journal : for cell and molecular biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 37 citations in OpenAlex.
- Systematic evaluation of the DNA repair machinery facilitated the development of efficient genome editing tools for the red yeastSynthetic and systems biotechnology · 2027Article
- Arabidopsis DNA repair mutants can integrate Agrobacterium T-DNA into the plant genome.The New phytologist · 2026Article
- Rational design of T-DNA vectors enables predictable, single-copy integration inbioRxiv : the preprint server for biology · 2026Article
- Efficient site-specific gene addition using R2 retrotransposons in tobacco and rice.Nature biotechnology · 2026Article
- Molecular regulators of regeneration and strategies for overcoming genotype-dependent recalcitrance in wheat (Triticum aestivum).Communications biology · 2026Review
- Stabilisation of Tomato Yellow Leaf Curl China Virus Infectious Clones Through Micro-Homology Mediated End Joining.Plant biotechnology journal · 2026Article
- TONSOKU prevents the formation of large tandem duplications and restrains ATR-WEE1 checkpoint activation.Nature communications · 2026Article
- EnhancedPlants (Basel, Switzerland) · 2025Article
- The evolving landscape of precise DNA insertion in plants.Nature communications · 2025Review
- Advances and prospects of large DNA fragment editing in plants.Nature plants · 2025Review
- Binary vector origin predictably determinesbioRxiv : the preprint server for biology · 2025Article
- Review
- Article
- A review of factors affecting the success of geminivirus infectious clones.Plant cell reports · 2025Review
- T-DNAreader: fast and precise identification of T-DNA insertion sites in plant genomes using RNA sequencing data.Genome biology · 2025Article
- Enhancing biolistic plant transformation and genome editing with a flow guiding barrel.Nature communications · 2025Article
- SeedSeg: image-based transgenic seed counting for segregation analysis of T-DNA loci.Plant methods · 2025Article
- Review
- Development of an RNA virus vector for non-transgenic genome editing in tobacco and generation ofaBIOTECH · 2024Article
- An efficient multiplex approach to CRISPR/Cas9 gene editing in citrus.Plant methods · 2024Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Attenuated strains of the naturally occurring plant pathogen Agrobacterium tumefaciens can transfer virtually any DNA sequence of interest to model plants and crops. This has made Agrobacterium-mediated transformation (AMT) one of the most commonly used tools in agricultural biotechnology. Understanding AMT, and its functional consequences, is of fundamental importance given that it sits at the intersection of many fundamental fields of study, including plant-microbe interactions, DNA repair/genome stability, and epigenetic regulation of gene expression. Despite extensive research and use of AMT over the last 40 years, the extent of genomic disruption associated with integrating exogenous DNA into plant genomes using this method remains underappreciated. However, new technologies like long-read sequencing make this disruption more apparent, complementing previous findings from multiple research groups that have tackled this question in the past. In this review, we cover progress on the molecular mechanisms involved in Agrobacterium-mediated DNA integration into plant genomes. We also discuss localized mutations at the site of insertion and describe the structure of these DNA insertions, which can range from single copy insertions to large concatemers, consisting of complex DNA originating from different sources. Finally, we discuss the prevalence of large-scale genomic rearrangements associated with the integration of DNA during AMT with examples. Understanding the intended and unintended effects of AMT on genome stability is critical to all plant researchers who use this methodology to generate new genetic variants.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.