Feasibility of NAD(P)/NAD(P)H as redox agents in enzymatic plasmonic gold nanostar assays for galactose quantification.

Tozivepi Aaron Munyayi, Danielle Wingrove Mulder, Engela Helena Conradie, Barend Christiaan Vorster

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Article in Royal Society open science, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 authors.

Tozivepi Aaron MunyayiCentre For Human Metabolomics, Department of Biochemistry, North West University Potchefstroom, 11 Hoffman Street, Potchefstroom 2531, South Africa.ORCID 0000-0001-7872-6133

Danielle Wingrove MulderCentre For Human Metabolomics, Department of Biochemistry, North West University Potchefstroom, 11 Hoffman Street, Potchefstroom 2531, South Africa.ORCID https://orcid.org/0000-0002-6970-7392

Engela Helena ConradieCentre For Human Metabolomics, Department of Biochemistry, North West University Potchefstroom, 11 Hoffman Street, Potchefstroom 2531, South Africa.ORCID https://orcid.org/0000-0002-1000-7711

Barend Christiaan VorsterCentre For Human Metabolomics, Department of Biochemistry, North West University Potchefstroom, 11 Hoffman Street, Potchefstroom 2531, South Africa.ORCID https://orcid.org/0000-0003-2371-288X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Plasmonic colorimetric sensors have emerged as powerful analytical tools in biochemistry due to their localized surface plasmon resonance extinction in the visible range. Here, we describe the feasibility of NAD(P)/NAD(P)H as redox agents in enzymatic plasmonic gold nanostar (AuNS) assays for galactose quantification using three model enzymes, GalDH, AR and GalOx, immobilized separately on polyvinylpyrrolidone-capped AuNS scaffolds. These highly specific, sensitive and selective bioassays induce the transformation of AuNS into quasi-spherical nanoparticles during the biorecognition of galactose in water and synthetic blood matrices. As a result, using our inexpensive and simple AuNS plasmon bioassays, the presence of galactose may be detected spectrophotometrically and by the naked eye.

Indexed as

bioassaybiorecognitionbiosensorgold nanostarsimmobilizedredox agents

Identifiers

PMID37830025

DOI10.1098/rsos.230825

PMCPMC10565372

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LicenceCC BY

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.