Improved preculture management for Cupriavidus necator cultivations.

Michelle-Sophie Gerlach, Peter Neubauer, Matthias Gimpel

Open access · hybridAbstract read

In one paragraph

Article in Biotechnology letters, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

0.1field-weighted citation impact, top 63% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

Glycerol-supplemented medium promotes transition of Cupriavidus necator from heterotrophic to lithoautotrophic growth.Biotechnology letters · 2025
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Michelle-Sophie GerlachChair of Bioprocess Engineering, Institute of Biotechnology, Technische Universität Berlin, Ackerstr. 76, ACK24, 13355, Berlin, Germany.

Peter NeubauerChair of Bioprocess Engineering, Institute of Biotechnology, Technische Universität Berlin, Ackerstr. 76, ACK24, 13355, Berlin, Germany.

Matthias GimpelChair of Bioprocess Engineering, Institute of Biotechnology, Technische Universität Berlin, Ackerstr. 76, ACK24, 13355, Berlin, Germany. matthias.gimpel@tu-berlin.de.ORCID http://orcid.org/0000-0002-5136-8169

Technische Universität Berlin · DE

Funding

German research foundation EXC 2008/1-390540038

6 · The paper itself

Abstract

objectivesResearch on hydrogenases from Cupriavidus necator has been ongoing for more than two decades and still today the common methods for culture inoculation are used. These methods were never adapted to the requirements of modified bacterial strains, resulting in different physiological states of the bacteria in the precultures, which in turn lead prolonged and different lag-phases.

resultsIn order to obtain uniform and always equally fit precultures for inoculation, we have established in this study an optimized protocol for precultures of the derivative of C. necator HF210 (C. necator HP80) which is used for homologous overexpression of the genes for the NAD

conclusionDespite having two subsequent precultures our protocol reduces the preculture time to less than 30 h and provides reproducible precultures for cultivation of C. necator HP80.

Indexed as

Cupriavidus necatorHydrogenaseCulture MediaFructoseNitrogenCulture MediaFructoseHydrogenaseNitrogenBioprocess developmentCupriavidus necatorNAD+-reducing hydrogenasePreculture management

Identifiers

PMID37828291

DOI10.1007/s10529-023-03436-1

PMCPMC10635987

OpenAlexW4387566815

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.