Evidence map›Paper›PMID 37828080›Full record

ArticleScientific reports2023

Rapid and visual detection of Toxoplasma gondii oocyst in cat feces using loop-mediated isothermal amplification (LAMP) assay.

Lingwei Sheng, Qiqi Xue, Sijia Xu, Fang Can, Ning Yao, Minghui Zou, Qiao Teng, Yuanyuan Li, Saeed El-Ashram, Yongsheng Ji and 1 more

Abstract read
In one paragraph

Article in Scientific reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

  1. Review
  2. Article
  3. PCR detection ofFood and waterborne parasitology · 2026
    Article
  4. Article
  5. Review
  6. Evolution ofJournal of parasitology research · 2026
    Review
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Lingwei ShengMedical Laboratory Science, Wannan Medical College, Wuhu, 241002, Anhui, China.
Qiqi XueDepartment of Medical Parasitology, Wannan Medical College, Wuhu, 241002, Anhui, China.
Sijia XuMedical Laboratory Science, Wannan Medical College, Wuhu, 241002, Anhui, China.
Fang CanSchool of Public Health, Wannan Medical College, Wuhu, 241002, Anhui, China.
Ning YaoSchool of Public Health, Wannan Medical College, Wuhu, 241002, Anhui, China.
Minghui ZouDepartment of Medical Parasitology, Wannan Medical College, Wuhu, 241002, Anhui, China.
Qiao TengDepartment of Medical Parasitology, Wannan Medical College, Wuhu, 241002, Anhui, China.
Yuanyuan LiDepartment of Medical Parasitology, Wannan Medical College, Wuhu, 241002, Anhui, China.
Saeed El-AshramCollege of Life Science and Engineering, Foshan University, Foshan, 528231, Guangdong, China.
Yongsheng JiDivision of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230026, Anhui, China. jiys2020@ustc.edu.cn.
Jinhong ZhaoDepartment of Medical Parasitology, Wannan Medical College, Wuhu, 241002, Anhui, China. zhaojh@wnmc.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Toxoplasma gondii is an obligate parasitic protozoon that transmits to animals and humans via ingested food. Cats that act as T. gondii's final hosts play a critical role in T. gondii transmission by shedding millions of oocysts. Timely diagnosis of infected cats is essential for preventing toxoplasmosis because oocysts are a putative T. gondii source in epidemiology. We developed a new visual LAMP assay targeting the B1 gene to analyze single oocysts in cat feces in this study. The amplification result could be visually estimated based on the color change. LAMP assay analytical sensitivity was 10

Indexed as

Cat DiseasesToxoplasmaAnimalsCatsDNA, ProtozoanFecesHumansMolecular Diagnostic TechniquesNucleic Acid Amplification TechniquesOocystsDNA, Protozoan

Identifiers

PMID37828080
PMCPMC10570283

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.