ArticleNature communications2023
Widefield imaging of rapid pan-cortical voltage dynamics with an indicator evolved for one-photon microscopy.
Article in Nature communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 30 citations in OpenAlex.
- Sub-millivolt voltage imaging reveals gap junction-mediated bioelectric contact inhibition.Nature communications · 2026Article
- Crosstalk-free optical indicator-actuator combinations for bidirectional optogenetics.npj biomedical innovations · 2026Review
- Behavior Differentially Shapes Spontaneous Cortical Network Dynamics Across Frequencies.bioRxiv : the preprint server for biology · 2026Article
- A red-emitting, genetically encoded indicator for two-photon voltage recording in vivo.bioRxiv : the preprint server for biology · 2026Article
- Causal and directional elements of global brain dynamics.bioRxiv : the preprint server for biology · 2026Article
- Intrinsic space-time couplings governing multi-scale cortical dynamics.bioRxiv : the preprint server for biology · 2026Article
- Designer indicators for two-photon recording of subthreshold voltage dynamics.Nature methods · 2026Article
- Designer indicators for two-photon recording of subthreshold voltage dynamics.bioRxiv : the preprint server for biology · 2026Article
- A versatile, positive-going voltage indicator that enables accessible two-photon recordings in vivo.bioRxiv : the preprint server for biology · 2026Article
- Voltage imaging as a window into neural computation.Neurophotonics · 2026Article
- Article
- An integrated platform for simultaneous wide-field voltage/calcium imaging and fMRI (EPI & ZTE) reveals neuronal infraslow dynamics underlying functional connectivity.bioRxiv : the preprint server for biology · 2026Article
- Noise Sources and Strategies for Signal Quality Improvement in Biological Imaging: A Review Focused on Calcium and Cell Membrane Voltage Imaging.Biosensors · 2026Review
- Voltage imaging with periodic structured illumination.Biomedical optics express · 2026Article
- Absolute Membrane Potential Recording with ASAP-Type Genetically Encoded Voltage Indicators Using Fluorescence Lifetime Imaging.ACS chemical neuroscience · 2025Article
- State-of-the-art preclinical techniques to study the impact of spreading depolarizations in awake rodents.The journal of headache and pain · 2025Review
- High-speed voltage imaging of action potentials in molecular layer interneurons reveals sensory-driven synchrony that augments movement.Cell reports · 2025Article
- Voltage Imaging with Periodic Structured Illumination.bioRxiv : the preprint server for biology · 2025Article
- Multiphoton Neurophotonics: Recent Advances in Imaging and Manipulating Neuronal Circuits.ACS photonics · 2025Review
- Impact of uniform illumination in widefield microscopy and mesoscopy.Scientific reports · 2025Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Widefield imaging with genetically encoded voltage indicators (GEVIs) is a promising approach for understanding the role of large cortical networks in the neural coding of behavior. However, the limited performance of current GEVIs restricts their deployment for single-trial imaging of rapid neuronal voltage dynamics. Here, we developed a high-throughput platform to screen for GEVIs that combine fast kinetics with high brightness, sensitivity, and photostability under widefield one-photon illumination. Rounds of directed evolution produced JEDI-1P, a green-emitting fluorescent indicator with enhanced performance across all metrics. Next, we optimized a neonatal intracerebroventricular delivery method to achieve cost-effective and wide-spread JEDI-1P expression in mice. We also developed an approach to correct optical measurements from hemodynamic and motion artifacts effectively. Finally, we achieved stable brain-wide voltage imaging and successfully tracked gamma-frequency whisker and visual stimulations in awake mice in single trials, opening the door to investigating the role of high-frequency signals in brain computations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.