Evidence map›Paper›PMID 37824076›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Differential Poly(A) Tail Length Analysis Using Nanopore Sequencing.

Showkat A Dar, Sulochan Malla, Cedric Belair, Manolis Maragkakis

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.8field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Showkat A DarLaboratory of Genetics and Genomics, National Institute on Aging, Intramural Research Program, National Institutes of Health, Baltimore, MD, USA.
Sulochan MallaLaboratory of Genetics and Genomics, National Institute on Aging, Intramural Research Program, National Institutes of Health, Baltimore, MD, USA.
Cedric BelairLaboratory of Genetics and Genomics, National Institute on Aging, Intramural Research Program, National Institutes of Health, Baltimore, MD, USA.
Manolis MaragkakisLaboratory of Genetics and Genomics, National Institute on Aging, Intramural Research Program, National Institutes of Health, Baltimore, MD, USA. emmanouil.maragkakis@nih.gov.
Institute on Aging · USNational Institute on Aging · US

Funding

RNA dynamics and co-translational decay in aging and aging-related diseaseZIAAG000696 · NIA · NATIONAL INSTITUTE ON AGING · PI MARAGKAKIS, MANOLIS · 2020 to 2025
$14.0M
Intramural NIH HHS ZIA AG000696
6 · The paper itself

Abstract

The poly(A) tail is a sequence of several adenosine nucleotides added to the 3' end of RNA molecules transcribed by polymerase II. The dynamics of poly(A) tail length play a significant role in regulating post-transcriptional gene expression by regulating the stability, translation, and decay of messenger RNAs. As a result, an accurate measurement of poly(A) tail length changes is important for understanding its regulatory function in different cellular contexts. Here, we outline a method for using nanopore sequencing and linear mixed models to analyze differences in poly(A) tail length across conditions.

Indexed as

Nanopore SequencingNucleotidesNucleotidyltransferasesPoly ARNA, MessengerNucleotidesNucleotidyltransferasesPoly ARNA, MessengerDeadenylationDifferential analysisNanopLenNanopore direct RNA sequencingPoly(A) tail

Identifiers

PMID37824076
OpenAlexW4387563670

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.